国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2015, Vol. 34 ›› Issue (5): 361-363.

• 论著 • 上一篇    下一篇

黄芪多糖对人精子冷冻后DNA完整性的保护作用

柳立军，马鹏程，王磊，张爱萍，王燕超，王晶   

  1. 730000 兰州大学第一医院人类精子库（柳立军，马鹏程，张爱萍，王燕超）；兰州大学生命科学学院发育生物学研究所（王磊）；甘肃中医药大学医学技术学院（王晶）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-09-15 发布日期:2015-09-15
  • 通讯作者: 王晶

Protective Effect of Astragalus Polyose on DNA Integrity of Human Sperm

LIU Li-jun，MA Peng-cheng，WANG Lei，ZHANG Ai-ping，WANG Yan-chao，WANG Jing   

  1. Human Sperm Bank，The First Hospital of Lanzhou University，Lanzhou 730000，China（LIU Li-jun，MA Peng-cheng，ZHANG Ai-ping，WANG Yan-chao）；Institute of Developmental Biology，School of Life Science, Lanzhou University，Lanzhou 730000，China（WANG Lei）；School of Medical Examination，Gansu University of Traditional Chinese Medicine，Lanzhou 730000，China（WANG Jing）
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-09-15 Online:2015-09-15
  • Contact: WANG Jing

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摘要： 目的：研究黄芪多糖（AP）对冻融人精子DNA完整性的保护作用。方法：收集正常（7份）和低活力（5份）共12例患者的精液标本，分别用含0，10，25，50 mg/L AP和1 g/L维生素E（VE）的冷冻保护液冻存精液，经过常规冻融和吖啶橙染色后，在荧光显微镜下计算精子的DNA碎片指数（DFI）。结果：在正常精子组中，T1（含10 mg/L AP）样本的DFI值比T0（含0 mg/L AP）、T2（含25 mg/L AP）、T3（含50 mg/L AP）和T4（含1 g/L VE）样本降低（P＜0.05）；在低活力精子组中，T1样本的DFI值低于T0和T4样本，差异有统计学意义（P＜0.05）。结论：AP在适当浓度时（10 mg/L）对冷冻保存的人精子DNA完整性有保护作用。

关键词: 黄芪多糖, 精液保存, 低温防护剂, DNA碎片裂

Abstract: Objective: To examine the protective effect of astragalus polyose (AP) on the DNA integrity of human sperm underwent freezing-thawing. Methods: The human semen samples with normal motility (7 samples) or low motility (5 samples) were collected. After grouped, the sperm suspension were freezed with the cryoprotective medium contained 0, 10, 25 and 50 mg/L of AP (the T0, T1, T2, T3 groups). The positive control is 1 g/L of Vitamin E (the T4 group). After the conventionally freezing-thawing, and dyeing with acridine orange, those sperm samples were detected the DNA fragmentation index (DFI) using fluorescence microscope. Results: In those groups of normal sperm motility, the DFI in the T1 group was significantly lower than those in the T0, T2, T3 and T4 groups (P＜0.05). In those groups of low sperm motility, the DFI in the T1 group was significantly lower than those in the T0 group and the T4 group (P＜0.05). Conclusions: The AP at 10 mg/L concentration has a protective effect on the DNA integrity of human sperm underwent freezing-thawing.

Key words: Astragalan, Semen preservation, Cryoprotective agents, DNA Fragmentation