国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2016, Vol. 35 ›› Issue (1): 9-12.

• 论著 • 上一篇    下一篇

PCNA、Survivin蛋白在人绒癌细胞株BeWo合体化过程中

易莉莎，林耀华，张国正   

  1. 510623 广州市妇女儿童医疗中心妇产科（易莉莎，张国正）；南华大学附属第一医院妇产科（林耀华）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: 张国正

Expressions of PCNA and Survivin Proteins in BeWo Cells during Syncytial Process

YI Li-sha，LIN Yao-hua，ZHANG Guo-zheng   

  1. Department of Obstetrics and Gynecology，Guangzhou Women and Children′s Medical Center，Guangzhou 510623，China（YI Li-sha，ZHANG Guo-zheng）；Department of Obstetrics and Gynecology，The First Affiliated Hospital of Nanhua University，Hengyang 421001，Hunan Province，China（LIN Yao-hua）
  • Received:1900-01-01 Revised:1900-01-01 Published:2016-01-15 Online:2016-01-15
  • Contact: ZHANG Guo-zheng

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摘要： 目的：通过检测人绒癌细胞株BeWo合体化过程中增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）、生存素（Survivin）蛋白表达的变化，探讨滋养细胞合体化后增殖性的变化，为恶性滋养细胞肿瘤，尤其是耐药恶性滋养细胞肿瘤的临床治疗提供新的思路和方法。方法：利用毛喉素（forskolin）诱导BeWo细胞株融合；应用逆转录聚合酶链反应（RT-PCR）检测促融素（Syncytin）在forskolin作用不同时间的BeWo细胞株中的表达；应用蛋白质印迹（Western blotting）检测PCNA、Survivin蛋白在forskolin作用不同时间的BeWo细胞株中的表达；应用噻唑蓝比色分析实验（MTT）法检测forskolin作用不同时间的绒癌细胞株BeWo的增殖能力。结果：①forskolin作用后的BeWo细胞株Syncytin基因的表达增强，且随着forskolin作用时间的延长，Syncytin的表达更强，于48 h达到高峰。②forskolin作用后的BeWo细胞株PCNA、Survivin蛋白的表达降低。③forskolin作用后的BeWo细胞株的增殖能力下降，且不同作用时间的差异有统计学意义；forskolin作用的时间越长，BeWo细胞株增殖能力下降越明显。结论：人绒癌细胞株BeWo合体化后PCNA、Survivin蛋白的表达降低，说明人绒癌细胞株BeWo合体化后增殖性降低，推测诱导滋养细胞合体化可能对临床治疗恶性滋养细胞肿瘤具有一定作用。

关键词: 增殖细胞核抗原, 微管相关蛋白质类, 福司柯林, 细胞融合

Abstract: Objective：To observe the expression changes of PCNA and Survivin proteins in the choriocarcinoma cell line（ＢｅＷｏ） during its fusion, so as to explore the proliferation change after the fusion of trophoblast cells. The study could be helpful for developing a new therapy of gestational trophoblastic tumor （ＧＴＴ）, especially drug-resistant GTT. Methods：The intercellular fusion of the cultured BeWo cells was induced by forskolin. The expression of Syncytin mRNA in the treated BeWo cells was detected by RT-PCR, and the expressions of PCNA and Survivin proteins were detected by Western blotting. The proliferation of BeWo cells was detected by MTT. Results：①The expression of Syncytin gene in the BeWo cell treated with forskolin was increased with a time-dependent manner, which reached its peak in 48 hours. ②The expressions of PCNA and Survivin proteins in BeWo cell after treatment were significantly decreased. ③The proliferation of the BeWo cell treated with forskolin was significantly decreased with a time-dependent manner. Conclusions：The lowered expressions of PCNA and Survivin proteins in those fusion choriocarcinoma cells means the lowered proliferation, suggesting that the induced intercellular fusion of trophoblast cells may be helpful to treat those malignant GTT.

Key words: Proliferating cell nuclear antigen, Microtubule-associated proteins, Forskolin, Cell fusion