国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2020, Vol. 39 ›› Issue (1): 5-11.

• 论著 •    下一篇

苯并芘代谢产物BPDE的细胞毒性研究及五味子乙素在预防HTR-8/SVneo细胞损伤中的作用

梁婧，侯海燕，王梦，孙旸，陈亚琼   

  1. 102618 北京，中国中医科学院广安门医院南区（梁婧）；中国人民武装警察部队特色医学中心妇产科（侯海燕，王梦，孙旸，陈亚琼）；天津市职业与环境危害防制重点实验室（侯海燕，陈亚琼）
  • 收稿日期:2019-07-23 修回日期:2019-10-26 出版日期:2020-01-15 发布日期:2020-01-15
  • 通讯作者: 陈亚琼，E-mail：chenyq82@hotmail.com E-mail:liangjing19850903@163.com
  • 基金资助:
    国家自然科学基金面上项目（81273977）；天津市自然科学基金一般项目（12JCYBJC16200）

Study on the Cytotoxicity of BPDE and Protective Effect of Schizandrin B in HTR8/SVneo Cells

LIANG Jing, HOU Hai-yan, WANG Meng, SUN Yang, CHEN Ya-qiong   

  1. South Area of Guang′anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 102618, China （ＬＩＡＮＧ Ｊｉｎｇ）； Ｄｅｐａrtment of Obstetrics and Gynecology, Characteristic Medical Center of Chinese People′s Armed Police Force, Tianjin 300162, Chinａ （ＨOU Hai-yan, WANG Meng, SUN Yang, CHEN Ya-qiｏｎｇ）； Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Tianjin 300162, Chinａ （ＨＯU Hai-yan, CHEN Ya-qioｎｇ）
  • Received:2019-07-23 Revised:2019-10-26 Published:2020-01-15 Online:2020-01-15
  • Contact: ＣHEN Ya-qiong, E-mａｉｌ： ｃｈｅnyq82@hotmail.com E-mail:liangjing19850903@163.com

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摘要： 目的：探讨苯并芘[Benzo（a）pyrene，BaP]代谢产物二氢二醇环氧苯并芘[7,8 dihydrodiol 9,10 epoxidebenzｏ（ａ）pyrene，BPDE]对人绒毛膜外滋养细胞株HTR-8/SVneo的毒性效应及五味子乙素（Schisandrin B，Sch B）对BPDE诱导的HTR8/SVneo细胞损伤的保护作用。方法：以HTR-8/SVneo细胞为载体，构建BPDE对HTR-8/SVneo细胞的染毒模型和Sch B对HTR-8/SVneo细胞的保护模型，利用MTS细胞增殖实验检测不同浓度的BPDE单独给药和Sch B联合BPDE给药对HTR-8/SVneo细胞增殖的影响。结果：5 μmol/L BPDE即可使细胞活力显著下降，活细胞数仅占总细胞数的38.23%。经不同浓度的Sch B（0.625、1.25、2.5、5、10和20 μmol/L）预处理后再以BPDE干预，细胞活力较前明显上升，分别为40.11%、42.68%、46.47%、47.34%、61.37%和49.87%，其保护作用在0.625～10 μmol/L之间呈浓度依赖关系。结论：BPDE对HTR-8/SVneo细胞产生剂量依赖的毒性效应；Sch B（0.625～10 μmol/L）对BPDE诱导的细胞损伤有保护作用。

关键词: 苯并芘, 二羟二氢苯并芘类, 二氢二醇环氧苯并芘, 五味子乙素, 人绒毛膜外滋养细胞, 细胞毒性

Abstract: Objective：To explore the cytotoxicity of dihydrodiol benzobenzopyrene [7, 8 dihydrodiol 9,10 epoxidebenzo(a) pyrene , BPDE], a metabolite of Benzo(a) pyrene (BaP), in human choriotrophoblastic cell line (HTR-8/SVneo cell).  The protective effect of Schisandrin B (Sch B) on the cell injury induced by BPDE was also investigated. Methods: HTR-8/SVneo cells were used to construct a model of BPDE cytotoxity and a model of Sch B protection. The cell proliferation was tested by MTS in two groups: one group was treated by different concentrations of BPDE alone; another was treated by Sch B combined with BPDE administration. Results: 5 μmol/L of BPDE significantly reduced the cell viability, and the number of living cells was only 38.23% of the total cells. The cells were pretreated with different concentrations of Schisandrin B (0.625, 1.25, 2.5, 5, 10 and 20 μmol/L), and followed by BPDE. The cell viability increased significantly (40.11%, 42.68%, 46.47%, 47.34%, 61.37% and 49.87%, respectively), which suggested a dose-dependent manner of protective effect of Sch B from 0.625 μmol/L to 10 μmol/L. Conclusions: BPDE has a toxic effect on HTR-8/SVneo cells in a dose-dependent manner; while Sch B (0.625-10 μ mol/L) has a protective effect on the cell injury induced by BPDE.

Key words: Benzo(a)pyrene, Dihydroxydihydrobenzopyrenes, BPDE, Schizandrin B;, Human extravillous cytotrophyblast, Cytotoxicity