国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2010, Vol. 29 ›› Issue (3): 235-238.

• 综述 • 上一篇    下一篇

多重置换扩增技术在植入前遗传学诊断中的应用

彭兆锋;孙莹璞   

  1. 450052 郑州大学第一附属医院生殖中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-15 发布日期:2010-05-15

Multiple Displacement Amplification and It’s Application in Preimplantation Genetic Diagnosis

PENG Zhao-feng;SUN Ying-pu.   

  1. Reproductive Medical Center of the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2010-05-15 Online:2010-05-15

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摘要： 多重置换扩增技术（MDA）是基于环状滚动扩增的全基因组扩增技术，具有高扩增效率和高保真性等特点。尽管用于单细胞扩增时存在某些缺陷，但其在胚胎植入前遗传学诊断（PGD）实验研
究和临床应用中仍取得巨大进展。MDA 联合常规PCR技术已成功用于PGD 的临床诊断，实现了单次胚胎活检、单个胚胎细胞的性别、部分单基因病的同步诊断。

关键词: 植入前遗传学诊断, 全基因组扩增, 多重置换扩增

Abstract: The multiple replacement amplification technical（MDA）is a kind of the whole genome amplification（WGA）which based on strand displacement amplification. It has higher amplification efficiency rate and fidelity characteristics and so on. Although it is ued in the single cell amplification except a little defects, it has acquired great progress in the practice research and clinical application of preimplantation genetic diagnosis（PGD）. MDA has succeed in PGD when combined with standard PCR method. It makes master synchronism diagnosis of single test embryo biopsy, the single embryo cell gender and monogenic
disorder come ture. MDA has impelled rapid development of PGD technology.

Key words: Preimplantation Genetic Diagnosis, Whole genome amplification, Multiple displacement amplification