国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2010, Vol. 29 ›› Issue (3): 221-224.

• 论著 • 上一篇    下一篇

提高人类精液中圆形细胞数量测定的精确度

Cooper TG;Hellenkemper B   

  1. Centre of Reproductive Medicine and Andrology,University of Munster,Domagkstrasse 11,D-48149 Munster,Germany
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-15 发布日期:2010-05-15

Improving Precision in the Assessment of Round Cell Numbers in Human Semen

Cooper TG;Hellenkemper B   

  1. Centre of Reproductive Medicine and Andrology,University of Munster,Domagkstrasse 11,D-48149 Munster,Germany
  • Received:1900-01-01 Revised:1900-01-01 Published:2010-05-15 Online:2010-05-15

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摘要： 本研究的目的是提高用过氧化物酶试验检测精液中的白细胞时所得的圆形细胞数量的精确度。精液样本的圆形细胞浓度在（0.6~6）×106/mL之间，降低精液的稀释度，增加被测悬浮液的体积。1+5（1∶6）的稀释度适合于测量过氧化物酶活性，并能为细胞检测提供足够清晰的背景。在该稀释度下，测定Neubauer鄄改良精子计数板两边所有18 个网格中的细胞数量，额定细胞浓度为（1.9~3.3)×106/mL 的10 个样本中只有3 个样本的圆形细胞数≥400 个。由于更低浓度的精子稀释液不适合测定圆形细胞或其过氧化物酶反应产物，所以无法精确测量（抽样误差5%）参考下限值（1×106/mL）。结果表明，正是由于测定精液中的10 个样本中圆形细胞很难精确到1×106/mL，所以白细胞精液症临界值的确定一直存在诸多分歧。因此需要建立一些统计学上合理的参考限。

关键词: 圆形细胞, 过氧化酶, 精确度, 细胞数

Abstract: The aim of this study was to increase the precision of assessment of the number of round cells observed in the peroxidase test for detection of seminal leukocytes（granulocytes）. The dilution of semen was reduced and the volume of suspension examined was increased for semen samples containing between 0.6 and 6 million round cells per mL. A1+5（1∶6）dilution was compatible with measurable peroxidase activity and a sufficiently clear background for cell assessment. At this dilution，and with assessment of all 18 grids on both sides of the Neubauer-improved counting chamber，only three of the 10 samples （nominal cell concentrations of 1.9×10(6)-3.3×10(6) mL-1) presented 400 round cells or more. As lower seminal dilutions were incompatible with easy detection of round cells or their peroxidase reaction product，it was not possible to provide precise measurements（sampling error 5%）of the suggested lower reference limit of 1伊106 cells per mL. The results indicate that this poor precision of measuring 1×10(6) round cells per mL could explain the discrepant reports on the acceptability of the cut-off values for leukocytospermia. Such reference limits need to be established with statistically sound methods.

Key words: Cell number, Peroxidase, Precision, Round cells, Semen