国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2010, Vol. 29 ›› Issue (2): 69-71.

• 论著 • 上一篇    下一篇

CREM 基因与特发性生精障碍关系的研究

王炯;贾书花;陈云霞;武延隽;苗聪秀;王改琴   

  1. 046011 长治医学院附属和济医院妇产科（王炯）；长治医学院组胚教研室（贾书花，王改琴），免疫学教研室（陈云霞，武延隽）；长治医学院附属和平医院生殖遗传中心（苗聪秀）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-15 发布日期:2010-03-15

The Research of the Relationship Between the CREM Gene Mutation and the IdiopathicSpermatogenesis Impairment

WANG Jiong, JIA Shu-hua, CHEN Yun-xia, WU Yan-jun, MIAO Cong-xiu,
WANG Gai-qin   

  1. Department of gynecology and obstetrics, Heji Hospital, Changzhi Medical College, Changzhi 046011, China（WANG Jiong）; Histology and Embryology Teaching and Research, Changzhi Medical College, Changzhi 046000, China （JIA Shu-hua, WANG Gai-qin）; Immunology Teaching and Research, Changzhi Medical College, Changzhi 046000, China（CHEN Yun-xia，WU Yan-jun）; Reproductive Genetics Center, Heping Hospital,Changzhi Medical College, Changzhi 046000, China（MIAO Cong-xiu）
  • Received:1900-01-01 Revised:1900-01-01 Published:2010-03-15 Online:2010-03-15

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摘要： 目的：探讨环磷酸腺苷反应元件调节物（CREM）基因与男性不育症中特发性生精障碍的关系。方法：收集特发性无精子症和严重少精子症各20 例患者的外周血，收集具有正常生育能力志愿者20例外周血作为对照。进行外周血提取DNA、聚合酶链反应（PCR）扩增、单链构象多态性（SSCP），检测特发性生精障碍患者外周血中CREM基因。结果：在65％的无精子症和55％严重少精子症患者中检测出CREM基因的变异。结论：在人类，CREM 基因在精子的发生发育过程中起着至关重要的作用，CREM 基因参与人类精子的发生。

关键词: 环磷酸腺苷反应元件调节物基因, 特发性生精障碍, 男性不育

Abstract:

Objective：To research the relationship between the cyclic AMP-responsive element modulator (CREM) gene mutation and the idiopathic permatogenesis impairment in male infertility. Methods：The peripheral bloods were collected from 20 patients with azoospermia and 20 patients with serious oligospermia. The peripheral bloods of 20 volunteers with normal fertility were collected as control group. The CREM gene of patients with the idiopathic spermatogenesis impairment was detected by collecting the DNA of
the peripheral blood, PCR amplification and single-strand conformation polymorphism. Results：65% of the patients with azoospermia and 55% of the patients with serious oligospermia had the CREM gene mutation. Conclusion：CREM gene plays an important role in the process of sperm development for humans. CREM gene is involved in human spermatogenesis occurrence.

Key words: Cyclic AMP-responsive element moclulator gene, Idiopathic spermatogenesisimpairment, Male infertility