Abstract: As development of the assisted reproductive technique（ＡＲＴ）， ｐｒｅｉｍｐｌａｎｔａｔｉｏｎ ｇｅｎｅｔｉｃ ｄｉａｇｎｏｓｉｓ（ＰＧＤ） ｈａｄ ｂｅｅｎ ｍａｄｅ ｇｒｅａｔ ｐｒｏｇｒｅｓｓ． Ｔｈｅ ｅｍｅｒｇｅｎｃｅｄ ａｐｐｌｉｃａｔｉｏｎ ｏｆ ｆｌｕｏｒｅｓｃｅｎｔ ｉｎ ｓｉｔｕ ｈｙｂｒｉｄｉｚａｔｉｏｎ（ＦＩＳＨ） ｉｎ ｃｌｉｎｉｃａｌ ｒａｉｓｅｄ ｉｍｐｌａｎｔａｔｉｏｎ ｒａｔｅ ａｎｄ ｃｌｉｎｉｃａｌ ｇｅｓｔａｔｉｏｎ rate consumedly in IVF, and lowed the miscarry rate. Now, FISH is widely used in aneuploidy screening, chromosome construction/structure abnormality and sex?鄄linked genetic diseases. But, the long?鄄term safety of PGD is still under controversy. In this review, we introduced application of FISH in the field of PGD.

Key words: Embryo transfer, Fertilization in vitro, In situ hybridization, fluorescence, Preimplantation diagnosis, Sex determination（genetics）, Genetic screening