高通量转录组测序技术在评价子宫内膜容受性中的应用价值

doi:10.12280/gjszjk.20200190

摘要/Abstract

摘要：

良好的子宫内膜容受性是胚胎植入的关键因素,容受期子宫内膜组织的基因表达与容受性密切相关。通过高通量转录组测序技术可以获得月经不同时期子宫内膜基因表达图谱以及调控基因表达的微小RNA（miRNA）和长链非编码RNA（lncRNA）序列,结合生物信息学分析,可以更好地了解容受期子宫内膜特异基因表达,并为其调控机制的研究提供技术支持,对于不孕症患者以及反复植入失败患者的诊治具有非常重要的临床应用价值。对高通量转录组测序在子宫内膜容受性及胚胎植入方面的相关研究进行了综述。

关键词: 高通量核苷酸序列分析, 植入窗, 基因, 子宫内膜, 后成说, 遗传, 体外受精, 胚胎移植

Abstract:

The good endometrial receptivity is a key factor for embryo implantation. The gene expression profile of endometrial tissue cells in the receptive period is closely related to the endometrial receptivity. High-throughput transcriptome sequencing technology can obtain gene expression profiles of the endometrium at different periods of menstruation, as well as microRNA (miRNA) and long non-coding RNA (lncRNA) sequences that regulate gene expression. Combined with bioinformatics analysis, these profiles are very helpful for us to understand the expression of specific genes in the endometrium during the receptivity period, and provide technical support for the mechanism study. This high-throughput method may be used for the diagnosis and treatment of patients with infertility and repeated implantation failure. This review summarized the research progress of high-throughput transcriptome sequencing in the evaluation of endometrial receptivity and embryo implantation.

Key words: High-throughput nucleotide sequencing, Implantation window, Genes, Endometrium, Epigenesis, genetic, Fertilization in vitro, Embryo transfer

白塔吉, 何金英, 马玉珍. 高通量转录组测序技术在评价子宫内膜容受性中的应用价值[J]. 国际生殖健康/计划生育, 2021, 40(1): 49-52.

BAI Ta-ji, HE Jin-ying, MA Yu-zhen. Application of High-Throughput Transcriptome Sequencing in Evaluation of Endometrial Receptivity[J]. Journal of International Reproductive Health/Family Planning, 2021, 40(1): 49-52.

参考文献 27

[1]	Bassil R, Casper R, Samara N, et al. Does the endometrial receptivity array really provide personalized embryo transfer?[J]. J Assist Reprod Genet, 2018,35(7):1301-1305. doi: 10.1007/s10815-018-1190-9.
[2]	Paria BC, Song H, Dey SK. Implantation: molecular basis of embryo-uterine dialogue[J]. Int J Dev Biol, 2001,45(3):597-605.
[3]	Mahajan N. Endometrial receptivity array: Clinical application[J]. J Hum Reprod Sci, 2015,8(3):121-129. doi: 10.4103/0974-1208.165153.
[4]	Edgell TA, Evans J, Lazzaro L, et al. Assessment of potential biomarkers of pre-receptive and receptive endometrium in uterine fluid and a functional evaluation of the potential role of CSF3 in fertility[J]. Cytokine, 2018,111:222-229. doi: 10.1016/j.cyto.2018.08.026.
[5]	Tan J, Kan A, Hitkari J, et al. The role of the endometrial receptivity array (ERA) in patients who have failed euploid embryo transfers[J]. J Assist Reprod Genet, 2018,35(4):683-692. doi: 10.1007/s10815-017-1112-2.
[6]	Teder H, Koel M, Paluoja P, et al. TAC-seq: targeted DNA and RNA sequencing for precise biomarker molecule counting[J]. NPJ Genom Med, 2018,3:34. doi: 10.1038/s41525-018-0072-5.
[7]	Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics[J]. Nat Rev Genet, 2009,10(1):57-63. doi: 10.1038/nrg2484.
[8]	Chen G, Shi T, Shi L. Characterizing and annotating the genome using RNA-seq data[J]. Sci China Life Sci, 2017,60(2):116-125. doi: 10.1007/s11427-015-0349-4.
[9]	Altmäe S, Reimand J, Hovatta O, et al. Research resource: interactome of human embryo implantation: identification of gene expression pathways, regulation,and integrated regulatory networks[J]. Mol Endocrinol, 2012,26(1):203-217. doi: 10.1210/me.2011-1196.
[10]	Hu S, Yao G, Wang Y, et al. Transcriptomic changes during the pre-receptive to receptive transition in human endometrium detected by RNA-Seq[J]. J Clin Endocrinol Metab, 2014,99(12):E2744-E2753. doi: 10.1210/jc.2014-2155.
[11]	Altmäe S, Koel M, Võsa U, et al. Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers[J]. Sci Rep, 2017,7(1):10077. doi: 10.1038/s41598-017-10098-3.
[12]	Rekker K, Altmäe S, Suhorutshenko M, et al. A Two-Cohort RNA-seq Study Reveals Changes in Endometrial and Blood miRNome in Fertile and Infertile Women[J]. Genes (Basel), 2018,9(12):574. doi: 10.3390/genes9120574.
[13]	Guo F, Si C, Zhou M, et al. Decreased PECAM1-mediated TGF-β1 expression in the mid-secretory endometrium in women with recurrent implantation failure[J]. Hum Reprod, 2018,33(5):832-843. doi: 10.1093/humrep/dey022.
[14]	Huang C, Sun H, Wang Z, et al. Increased Krüppel-like factor 12 impairs embryo attachment via downregulation of leukemia inhibitory factor in women with recurrent implantation failure[J]. Cell Death Discov, 2018,4:23. doi: 10.1038/s41420-018-0088-8.
[15]	邢晶晶. Kisspeptin、GPR54和LIF在反复移植失败患者植入窗期子宫内膜表达的研究[D]. 郑州:郑州大学, 2017.
[16]	Shi C, Han HJ, Fan LJ, et al. Diverse endometrial mRNA signatures during the window of implantation in patients with repeated implantation failure[J]. Hum Fertil(Camb), 2018,21(3):183-194. doi: 10.1080/14647273.2017.1324180.
[17]	张倩. 非编码RNAs miR-148a-3p、lincARDD1在反复胚胎种植失败蜕膜化中的作用及机制研究[D]. 济南:山东大学, 2018.
[18]	Gross N, Kropp J, Khatib H. MicroRNA Signaling in Embryo Development[J]. Biology (Basel), 2017,6(3):34. doi: 10.3390/biology6030034.
[19]	Shi C, Shen H, Fan LJ, et al. Endometrial MicroRNA Signature during the Window of Implantation Changed in Patients with Repeated Implantation Failure[J]. Chin Med J (Engl), 2017,130(5):566-573. doi: 10.4103/0366-6999.200550.
[20]	Liang J, Wang S, Wang Z. Role of microRNAs in embryo implantation[J]. Reprod Biol Endocrinol, 2017,15(1):90. doi: 10.1186/s12958-017-0309-7.
[21]	Wang Q, Wang N, Cai R, et al. Genome-wide analysis and functional prediction of long non-coding RNAs in mouse uterus during the implantation window[J]. Oncotarget, 2017,8(48):84360-84372. doi: 10.18632/oncotarget.21031.
[22]	Sigurgeirsson B, Åmark H, Jemt A, et al. Comprehensive RNA sequencing of healthy human endometrium at two time points of the menstrual cycle[J]. Biol Reprod, 2017,96(1):24-33. doi: 10.1095/biolreprod.116.142547.
[23]	Chen MY, Liao GD, Zhou B, et al. Genome-Wide Profiling of Long Noncoding RNA Expression Patterns in Women With Repeated Implantation Failure by RNA Sequencing[J]. Reprod Sci, 2019,26(1):18-25. doi: 10.1177/1933719118756752.
[24]	Xu H, Zhou M, Cao Y, et al. Genome-wide analysis of long noncoding RNAs, microRNAs,and mRNAs forming a competing endogenous RNA network in repeated implantation failure[J]. Gene, 2019,720:144056. doi: 10.1016/j.gene.2019.144056.
[25]	Feng C, Shen JM, Lv PP, et al. Construction of implantation failure related lncRNA-mRNA network and identification of lncRNA biomarkers for predicting endometrial receptivity[J]. Int J Biol Sci, 2018,14(10):1361-1377. doi: 10.7150/ijbs.25081.
[26]	Patel JA, Patel AJ, Banker JM, et al. Personalized Embryo Transfer Helps in Improving In vitro Fertilization/ICSI Outcomes in Patients with Recurrent Implantation Failure[J]. J Hum Reprod Sci, 2019,12(1):59-66. doi: 10.4103/jhrs.JHRS_74_18.
[27]	Koot YE, van Hooff SR, Boomsma CM, et al. An endometrial gene expression signature accurately predicts recurrent implantation failure after IVF[J]. Sci Rep, 2016,6:19411. doi: 10.1038/srep19411.