不明原因胚胎停育绒毛和蜕膜组织芳香烃受体和细胞色素P4501A1蛋白的表达及意义

国际生殖健康/计划生育 ›› 2017, Vol. 36 ›› Issue (6): 451-453.

不明原因胚胎停育绒毛和蜕膜组织芳香烃受体和细胞色素P4501A1蛋白的表达及意义

王云凤，连立芬，侯海燕，梁婧，陈俊，陈亚琼，董渠龙

300193 天津中医药大学（王云凤，陈俊，陈亚琼）；中国人民武装警察部队后勤学院附属医院妇产科（王云凤，侯海燕，陈俊，陈亚琼，董渠龙）；秦皇岛市妇幼保健院（连立芬）；中国中医科学院广安门医院南区（梁婧）

收稿日期:2017-07-20 修回日期:2017-10-27 出版日期:2017-11-15 发布日期:2017-11-15
通讯作者: 陈亚琼，E-mail：chenyq82@hotmail.com E-mail:27475092@qq.com
作者简介:2017-07-31
基金资助:
国家自然科学基金（81273977）；天津市应用基础与前沿技术研究计划青年项目（15JCQNJC12300）

Expressions of AhR and CYP1A1 Protein in Chorionic and Decidual Tissues of Unexplained Embryonic Diapauses and Its Significance

WANG Yun-feng，LIAN Li-fen，HOU Hai-yan，LIANG Jing，CHEN Jun，CHEN Ya-qiong，DONG Qu-long

Tianjin University of Traditional Chinese Medicine，Tianjin 300193，China（WANG Yun-feng，CHEN Jun，CHEN Ya-qiong）；Department of Obstetrics and Gynecology，Affiliated Hospital of Logistics University of the Chinese People′s Armed Forces，Tianjin 300162，China（WANG Yun-feng，HOU Hai-yan，CHEN Jun，CHEN Ya-qiong，DONG Qu-long）；Maternity and Child Care Hospital of Qinhuangdao，Qinhuangdao 066000，China（LIAN Li-fen）；South of Guang′anmen Hospital，China Academy of Chinese Medical Sciences，Beijing 102618，China（LIANG Jing）

Received:2017-07-20 Revised:2017-10-27 Published:2017-11-15 Online:2017-11-15
Contact: CHEN Ya-qiong，E-mail：chenyq82@hotmail.com E-mail:27475092@qq.com

摘要/Abstract

摘要： 目的：探讨芳香烃受体（aryl hydrocarbon receptor，AhR）和细胞色素P4501A1（cytochrome P450 1A1，CYP1A1）蛋白在胚胎停育患者绒毛及蜕膜中的表达。方法：分别选取30例不明原因胚胎停育患者作为病例组和30例正常早期妊娠人工流产妇女作为对照组，采用免疫组织化学方法检测2组患者绒毛和蜕膜组织中AhR和CYP1A1蛋白表达水平。结果：绒毛组织中，病例组AhR蛋白表达水平（0.58±0.03）高于对照组（0.25±0.04），病例组CYP1A1蛋白表达水平（0.60±0.01）高于对照组（0.22±0.03），差异均有统计学意义（P＜0.05）。蜕膜组织中，病例组AhR蛋白表达水平（0.56±0.03）高于对照组（0.26±0.04），病例组CYP1A1蛋白表达水平（0.57±0.01）高于对照组（0.24±0.03），差异均有统计学意义（P＜0.05）。结论：胚胎停育患者绒毛和蜕膜组织中AhR和CYP1A1蛋白表达明显增高，可能与不明原因胚胎停育的发生有关。

关键词: 胚胎丢失, 蜕膜, 绒毛, 烃类, 芳香, 细胞色素P450 CYP1A1, 胚胎停育

Abstract: Objective：To test the expressions of aryl hydrocarbon receptor (AhR) and cytochrome P4501A1 (CYP1A1) protein in the chorionic and decidual tissues of embryonic diapause. Methods：30 cases of early unexplained embryonic diapauses (the study group) and 30 cases of induced abortion in normal early pregnancy (the control group) were selected. The expression levels of AhR and CYP1A1 in the chorionic tissues and decidual tissues were detected by immunohistochemical method. Results：The study group had significantly high expression of AhR protein in the chorionic tissues (0.58±0.03, P＜0.01) and the decidual tissues (0.56±0.03, P＜0.01) when compared with the control group (0.25±0.04, 0.26±0.04). Meanwhile, the study group had significantly high expression of CYP1A1 protein in the chorionic tissues (0.60±0.01, P＜0.01) and the decidual tissues (0.57±0.01, P＜0.01) when compared with the control group (0.22±0.03, 0.24±0.03). Conclusions：The increased expressions of AhR and CYP1A1 protein in the chorionic and decidual tissues may be related to the pathogenesis of embryonic diapauses.

Key words: Embryo loss, Decidua, Chorion, Hydrocarbons, aromatic, Cytochrome P-450 CYP1A1, Embryonic diapauses

王云凤，连立芬，侯海燕，梁婧，陈俊，陈亚琼，董渠龙. 不明原因胚胎停育绒毛和蜕膜组织芳香烃受体和细胞色素P4501A1蛋白的表达及意义[J]. 国际生殖健康/计划生育, 2017, 36(6): 451-453.

WANG Yun-feng，LIAN Li-fen，HOU Hai-yan，LIANG Jing，CHEN Jun，CHEN Ya-qiong，DONG Qu-long. Expressions of AhR and CYP1A1 Protein in Chorionic and Decidual Tissues of Unexplained Embryonic Diapauses and Its Significance[J]. Journal of International Reproductive Health/Family Planning, 2017, 36(6): 451-453.

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