CRISP2蛋白研究进展

doi:10.12280/gjszjk.20200672

摘要/Abstract

摘要：

CRISPs（cysteine rich secretory proteins）蛋白家族有4个成员,CRISP2是唯一在睾丸中表达的CRISPs成员,组织特异性较高。精母细胞表达和分泌CRISP2,不受雄激素的影响。CRISP2分布于生精上皮内,定位于精母细胞和支持细胞间的连接处,可以增强精母细胞连接到支持细胞上的能力;继而存在于圆形精子细胞及延长型精子细胞,可结合到精子尾部和头部顶体,并最终定位于精子尾部以及顶体。CRISP2可能与精子顶体RyR3受体结合调节Ca²⁺内流;此外,CRISP2可能还具有调节RyR受体之外的其他离子通道（如抑制K⁺通道）的功能。研究表明,CRISP2参与了精子成熟和获能的全程,也在精卵结合的过程中起一定作用。综述CRISP2蛋白在睾丸的表达和分布、调控以及作用。

关键词: CRISP2, 精卵结合, 精子卵子相互作用, 不育,男(雄)性, 弱精子症, TPX1

Abstract:

CRISPs (cysteine rich secretory proteins) family contains four members. CRISP2 is the only member expressed in testicles, with high tissue specificity. CRISP2 is expressed and secreted by spermatocytes. This process won′t be affected by androgen. CRISP2 scatters in the seminiferous epithelium and is located at the junction of spermatocytes and Sertoli cells, helping spermatocytes connect to Sertoli cells. Afterward, CRISP2 goes with round spermatids and elongated spermatids, combines to the tail and the acrosome in the head of spermatozoa. CRISP2 probably modulate the influx of Ca²⁺ by combining with RyR3. CRISP2 may also act in modulating other receptors like inhibiting K⁺ channels. Research indicates that CRISP2 participates in the maturation and capacitation of spermatozoon all along, and also works in sperm-oocyte fusion. In this review, we will focus on the expression and distribution of CRISP2 in testicles as well as its regulation and function.

Key words: CRISP2, Sperm-oocyte fusion, Sperm-ovum interactions, Infertility, male, Asthenozoospermia, TPX1

姚宇童, 祁丛, 迪娜·叶尔努尔, 吴诗琪, 马婕, 刘强. CRISP2蛋白研究进展[J]. 国际生殖健康/计划生育, 2021, 40(4): 323-327.

YAO Yu-tong, QI Cong, Dina Yeernuer, WU Shi-qi, MA Jie, LIU Qiang. Research Progress of CRISP2 Protein[J]. Journal of International Reproductive Health/Family Planning, 2021, 40(4): 323-327.

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