国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (5): 361-363.

• 论著 • 上一篇    下一篇

阴道分泌物常规检验室内质量控制结果分析

苏莉，周湘红，安邦权，黄盛文，凌晓午，许筑，许健，杨秀华   

  1. 550002 贵阳，贵州省人民医院输血科（苏莉，安邦权），检验科（周湘红，安邦权，黄盛文，凌晓午，许筑，杨秀华）；贵州省临床检验中心（许健）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 安邦权

Internal Quality Control of Vaginal Secretions Routine Test

SU Li，ZHOU Xiang-hong，AN Bang-quan，HUANG Sheng-wen，LING Xiao-wu，XU Zhu，XU Jian，YANG Xiu-hua   

  1. Department of Clinical Laboratory（ZHOU Xiang-hong，AN Bang-quan，HUANG Sheng-wen，LING Xiao-wu，XU Zhu，YANG Xiu-hua），Guizhou Provincial People′s Hospital，Guiyang 550002，China；Guizhou Provincial Center for Clinical Laboratory，Guiyang 550002，China（XU Jian）
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-09-15 Online:2014-09-15
  • Contact: AN Bang-quan

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摘要： 目的：分析阴道分泌物（白带）常规检验室内质量控制（IQC）效果。方法：收集2011年1月—12月到贵州省人民医院妇产科就诊患者白带标本1 235份，每天抽取5份悬浮法检测的白带标本，涂片用瑞氏染色镜检，可疑淋球菌时加革兰染色复检，以此对悬浮法检测结果进行质量考核。每月不定期采用直接镜检及离心沉渣涂片镜检抽查生理盐水质量。结果：2011年的12个月中，仅7月和9月的线索细胞的符合率不合格，分别为74.3%和78.1%，其他月份的各项目检测符合率均达到合格标准，即82.5%～100%，月平均90.2%～94.7%，为优良等次；病原体中滴虫的平均符合率最高（99.3%），线索细胞的平均符合率最低（83.1%）。生理盐水质量为合格。结论：采用瑞氏法为主的复查监督措施，建立的悬浮法白带常规检查IQC方法可有效保证其检验质量。

关键词: 白带, 阴道涂片, 毛滴虫, 阴道, 质量控制, 染色与标记

Abstract: Objective： To evaluate the internal quality control (IQC) of vaginal secretions （leucorrhea） routine testing. Methods：Total 1 235 samples were included in this study. Five samples were randomly selected every day from leucorrhea testing by suspension. The smears stained with Wright’s were conducted by microscopic examination. Those suspicious samples with positive neisseria gonorrhoeae were rechecked by Gram stain，to assess the quality of suspecsion assay. The quality of normal saline was monthly checked by microscopic examination in direct smear or centrifugal sediment. Results：Among 12 months in 2011, besides the compliance rates of clue cells only in July and September were not qualified, 74.3% and 78.1% respectively, those rates in other months matched the standard of 82.5%-100%，the monthly average 90.2%-94.7%，suggesting that the testing quality was excellent. The compliance rate of trichomonas was highest（99.3%） in pathogens，while the compliance rate of clue cells was lowest（83.1%）. The quality normal saline was eligible. Conclusions：The IQC of routine leucorrhea suspension testing, which established by Wright’s method，can effectively ensure the testing quality in clinical practice.

Key words: Leukorrhea, Vaginal smears, Trichomonas vaginalis, Quality control, Staining and labeling