Abstract: This article reviews the latest edition of the World Health Organization’s manual on semen analysis，a comprehensive instructional guide. The methodology used in the assessment of the usual variables in semen analysis is described，as are many of the less common，but very valuable，sperm function tests. Seminal fluid preparation techniques for procedures such as in vitro fertilization and intrauterine insemination are also outlined in the manual. In addition，it details many useful techniques for the assessment of seminal fluid. It will
be a very useful manual for any laboratory that carries out analyses of seminal fluid.

Key words: Laboratory practice, Male infertility, Semen analysis