Abstract: The manipulation of gametes and embryos in an in vitro environment when performing assisted reproductive techniques（ART）carries the risk of exposure of these cells to supraphysiological levels of reactive oxygen species（ROS）. The main objective of this review articles is to provide ART personnel with
all the necessary information regarding the development of oxidative stress in an ART setting，as well as the sources of ROS and the mechanisms of oxidative stress-induced damage during ART procedures. The impact of oxidative stress on ART outcomes and the different strategies designed to prevent it are also discussed.

Key words: Oxidative stress, Assisted reproductive techniques, Infertilitas feminis, Sperm, Oocyte