Abstract: Objective： To construct the recombinant adenovirus vector of Calretinin （Calb2） gene for over-expression of Calb2 gene，as useful tools to study the effects of Calb2 on the regulation of reproductive endocrinology，central nerve system，and visual conduction. Methods：The cDNA sequence of Calb2 was cloned by the reverse transcriptive polymerase chain reaction （RT-PCR）. A Calb2 gene fragment was sub-cloned into adenovirus shuttle plasmid pAdTrack-CMV to construct the shuttle plasmid pAdTrack-Calb2. Then it was transformed into BJ5183 cells with the adenoviral backbone pAdEasy-1 to obtain the homologous recombinant AdCMV-Calb2. The recombinant AdCMV-Calb2 was further packaged and amplificated in AD293 cells. The expression of Calb2 protein in AD293 cells was detected by Western blotting. Results：The Calb2 gene recombinant adenovirus vectors，AdCMV-Calb2，were constructed successfully by endonulease digestion and sequencing. The AD293 cells infected with AdCMV-Calb2 significantly expressed GFP protein. The expression of CALB2 protein was significantly up-regulated in AD293 cells infected with AdCMV-Calb2 plasmids. Conclusions： The recombinant adenovirus vectors of AdCMV-Calb2 were successfully constructed and their expression was identified in AD293，which has provided a laboratory basis to study the function of Calb2 gene.

Key words: Calb2 gene, Adenoviridae, Genetic vector, Urogenital system