Journal of International Reproductive Health/Family Planning

Journal of International Reproductive Health/Family Planning ›› 2020, Vol. 39 ›› Issue (1): 5-11.

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Study on the Cytotoxicity of BPDE and Protective Effect of Schizandrin B in HTR8/SVneo Cells

LIANG Jing, HOU Hai-yan, WANG Meng, SUN Yang, CHEN Ya-qiong   

  1. South Area of Guang′anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 102618, China （ＬＩＡＮＧ Ｊｉｎｇ）； Ｄｅｐａrtment of Obstetrics and Gynecology, Characteristic Medical Center of Chinese People′s Armed Police Force, Tianjin 300162, Chinａ （ＨOU Hai-yan, WANG Meng, SUN Yang, CHEN Ya-qiｏｎｇ）； Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazards, Tianjin 300162, Chinａ （ＨＯU Hai-yan, CHEN Ya-qioｎｇ）
  • Received:2019-07-23 Revised:2019-10-26 Published:2020-01-15 Online:2020-01-15
  • Contact: ＣHEN Ya-qiong, E-mａｉｌ： ｃｈｅnyq82@hotmail.com E-mail:liangjing19850903@163.com

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Abstract: Objective：To explore the cytotoxicity of dihydrodiol benzobenzopyrene [7, 8 dihydrodiol 9,10 epoxidebenzo(a) pyrene , BPDE], a metabolite of Benzo(a) pyrene (BaP), in human choriotrophoblastic cell line (HTR-8/SVneo cell).  The protective effect of Schisandrin B (Sch B) on the cell injury induced by BPDE was also investigated. Methods: HTR-8/SVneo cells were used to construct a model of BPDE cytotoxity and a model of Sch B protection. The cell proliferation was tested by MTS in two groups: one group was treated by different concentrations of BPDE alone; another was treated by Sch B combined with BPDE administration. Results: 5 μmol/L of BPDE significantly reduced the cell viability, and the number of living cells was only 38.23% of the total cells. The cells were pretreated with different concentrations of Schisandrin B (0.625, 1.25, 2.5, 5, 10 and 20 μmol/L), and followed by BPDE. The cell viability increased significantly (40.11%, 42.68%, 46.47%, 47.34%, 61.37% and 49.87%, respectively), which suggested a dose-dependent manner of protective effect of Sch B from 0.625 μmol/L to 10 μmol/L. Conclusions: BPDE has a toxic effect on HTR-8/SVneo cells in a dose-dependent manner; while Sch B (0.625-10 μ mol/L) has a protective effect on the cell injury induced by BPDE.

Key words: Benzo(a)pyrene, Dihydroxydihydrobenzopyrenes, BPDE, Schizandrin B;, Human extravillous cytotrophyblast, Cytotoxicity