Abstract: Objective: To investigate the chromosome abnormalities in early spontaneous abortion villi of artificial intrauterine insemination (AIH) pregnancy and natural pregnancy, as well as the effect of the related factors of assisted reproduction on above chromosome abnormalities. Methods：The clinical data of 104 patients with early spontaneous abortion in our hospital from January 2017 to December 2019 were retrospectively analyzed, including 27 in the AIH group and 77 in the natural pregnancy group. All abortion villous samples were tested for karyotype. The incidences of chromosome abnormalities in the two groups were compared. The effects of female age and AIH assisted pregnancy on the chromosome abnormalities were investigated. Results：The total rate of chromosome abnormalities in early abortion villi was 52.88% (55/104), of which the chromosome number abnormality accounted for 94.55% (52/55). There was no significant difference in the incidence of chromosome abnormalities between the two groups ( χ2=0.010, P=0.921). The patients in the two groups were sub-grouped according to their ages ＜25 years, 25-29 years, 30-34 years and ≥35 years, individually. There was significant difference in the rate of villous chromosome abnormalities among the aged-subgroups of the AIH group and the natural pregnancy group (all P＜0.05). However, there were no significant differences between the corresponding aged-subgroups of the two groups(P=1.00). In the 27 cases of AIH early abortion, 15 cases had villous chromosome abnormalities. Conclusions：AIH does not increase the chromosome abnormalities in early spontaneous abortion villi, and patients with spontaneous abortion should promptly find the causes of abortion. AIH can be recommended for those infertile patients with the AIH-related indications.

Key words: Abortion, spontaneous；, Chorionic villi；, Chromosome aberrations；, Karyotyping；, Insemination, artificial；, Pregnancy