A Case of Successful Pregnancy and Delivery after ICSI Derived from Zona-Free Oocyte

doi:10.12280/gjszjk.20200181

Abstract

Abstract:

Zona-free oocytes (ZFO) are frequently encountered in in-vitro fertilization (IVF) laboratories. The majority of these ZFO are due to the damage during manipulation, rather than abnormal zona production. We reported data case of a single live birth healthy baby after blastocyst transplantation following ICSI insemination of a naked egg after zona pellucida shedding. The biological function of zona pellucida and the clinical value of ZFOs were reviewed and discussed. We suggest that those mature and morphologically normal ZFO may be rescued, fertilized with ICSI and then cultured to the blastocyst stage for subsequent transfer or cryopreservation, so as to provide valuable embryo sources for infertile patients, especially for those patients with limited oocytes.

Key words: Sperm injections, intracytoplasmic, Live birth, Pregnancy, Zone-free oocyte, Zone-free blastocyst

ZHANG Hui, LU Jing, JING Li, CHAI Liang-ting. A Case of Successful Pregnancy and Delivery after ICSI Derived from Zona-Free Oocyte[J]. Journal of International Reproductive Health/Family Planning, 2021, 40(1): 29-31.

Figures/Tables 1

References 19

[1]	Shu Y, Peng W, Zhang J. Pregnancy and live birth following the transfer of vitrified-warmed blastocysts derived from zona- and corona-cell-free oocytes[J]. Reprod Biomed Online, 2010,21(4):527-532. doi: 10.1016/j.rbmo.2010.04.033.
[2]	Hiraoka K, Hiraoka K, Kinutani M, et al. Case report: successful pregnancy after vitrification of a human blastocyst that had completely escaped from the zona pellucida on day 6[J]. Hum Reprod, 2004,19(4):988-990. doi: 10.1093/humrep/deh177.
[3]	Stanger JD, Stevenson K, Lakmaker A, et al. Pregnancy following fertilization of zona-free, coronal cell intact human ova: Case Report[J]. Hum Reprod, 2001,16(1):164-167. doi: 10.1093/humrep/16.1.164.
[4]	Ding J, Rana N, Dmowski WP. Intracytoplasmic sperm injection into zona-free human oocytes results in normal fertilization and blastocyst development[J]. Hum Reprod, 1999,14(2):476-478. doi: 10.1093/humrep/14.2.476.
[5]	Hu Y, Trolice MP. Live birth of twins derived from zona-free oocytes[J]. Fertil Steril, 2016,105(5):1232-1235. doi: 10.1016/j.fertnstert.2016.01.023.
[6]	Vajta G, Rienzi L, Bavister BD. Zona-free embryo culture: is it a viable option to improve pregnancy rates?[J]. Reprod Biomed Online, 2010,21(1):17-25. doi: 10.1016/j.rbmo.2010.03.014.
[7]	Gupta SK. The Human Egg′s Zona Pellucida[J]. Curr Top Dev Biol, 2018,130:379-411. doi: 10.1016/bs.ctdb.2018.01.001.
[8]	Suzuki H, Togashi M, Adachi J, et al. Developmental ability of zona-free mouse embryos is influenced by cell association at the 4-cell stage[J]. Biol Reprod, 1995,53(1):78-83. doi: 10.1095/biolreprod53.1.78.
[9]	Nichols J, Gardner RL. Effect of damage to the zona pellucida on development of preimplantation embryos in the mouse[J]. Hum Reprod, 1989,4(2):180-187. doi: 10.1093/oxfordjournals.humrep.a136868.
[10]	Yumoto K, Shimura T, Mio Y. Removing the zona pellucida can decrease cytoplasmic fragmentations in human embryos: a pilot study using 3PN embryos and time-lapse cinematography[J]. J Assist Reprod Genet, 2020,37(6):1349-1354. doi: 10.1007/s10815-020-01773-y.
[11]	Sampaío MA, Geber S. Births after transfer of zona-free blastocysts in oocyte donation cycles[J]. J Assist Reprod Genet, 2001,18(3):156-159. doi: 10.1023/a:1009407903447.
[12]	Urman B, Balaban B, Alatas C, et al. Zona-intact versus zona-free blastocyst transfer: a prospective,randomized study[J]. Fertil Steril, 2002,78(2):392-396. doi: 10.1016/s0015-0282(02)03238-7.
[13]	Syrkasheva AG, Dolgushina NV, Romanov AY, et al. Cell and genetic predictors of human blastocyst hatching success in assisted reproduction[J]. Zygote, 2017,25(5):631-636. doi: 10.1017/S0967199417000508.
[14]	Kirienko KV, Apryshko VP, Naumova AA, et al. Mechanical zona pellucida removal of vitrified-warmed human blastocysts does not affect the clinical outcome[J]. Reprod Biomed Online, 2019,39(5):745-749. doi: 10.1016/j.rbmo.2019.06.003.
[15]	Hsieh YY, Chang CC, Tsai HD. Zona-free oocyte fertilized with intracytoplasmic sperm injection and underwent further division: case report and literature review[J]. Chang Gung Med J, 2001,24(9):599-602.
[16]	Takahashi K, Araki Y, Motoyama M. Normal development of a zona-free oocyte to the blastocyst stage following ICSI[J]. Hum Reprod, 1999,14(10):2677. doi: 10.1093/humrep/14.10.2677.
[17]	Bodri D, Kato R, Kondo M, et al. Time-lapse monitoring of zona pellucida-free embryos obtained through in vitro fertilization: a retrospective case series[J]. Fertil Steril, 2015,103(5):e35. doi: 10.1016/j.fertnstert.2015.02.016.
[18]	Ueno S, Bodri D, Uchiyama K, et al. Developmental potential of zona pellucida-free oocytes obtained following mild in vitro fertilization[J]. Fertil Steril, 2014,102(6):1602-1607. doi: 10.1016/j.fertnstert.2014.08.025.
[19]	马超, 邵小光, 金波, 等. 胞质完整的无透明带卵母细胞临床利用价值探讨[J]. 生殖医学杂志, 2018,27(1):32-36. doi: 10.3969/j.issn.1004-3845.2018.01.007.