Abstract: Granulosa is important in the nutrition and signal transduction for oocyte development andmaturation. Similarly，oocyte also plays regulating roles in the granulosa differentiation and development. Theprofile of gene expression of granulosa may be used to evaluate the micro-environment of oocyte development.The patterns of gene expression of granulosa may be associated with the oocyte quality. Therefore，it is possibleto identify the markers of oocyte and embryo competence by analyzing the granulosa transcriptome, by whichthe IVF fertilization rate, implantation rate and pregnancy rate could be increased, and the miscarriage ratedecreased.

Key words: Oocytes, Ovarian follicle, Fertilization in vitro, Transcription, genetic