Abstract: It is important to get the mature sperm with cellular structure and DNA intact and non-apoptosis for high ART pregnancy rate. The electrophoresis microflew sperm sorter based on sperm size andsurface charge can be used to get quickly the membrane mature sperm. The non -apoptotic sperm can beselected by the outer surface of sperm membrane phosphatidylserine externalization binding with Annexin-V-conjugated paramagnetic microbeads. The formation of hyaluronic-acid (HA)-binding sites on sperm membraneis one of signs of sperm maturation, by which the sperm of membrane mature can be selected. The ultra-microscope provides convenience to distinguish subcellular organelles of sperm. SperMagic hasa unique role inpromoting sperm motility and improving sperm fertilizing capacity ,and consequently increases pregnancy rate.Compared with the routine density gradient centrifugation method, above methods to deal with sperm will resultin different fertilization rate ,embryo quality and pregnancy rate. More efforts to improve ART pregnancyoutcome will still be how to get the ideal sperm.

Key words: Spermatozoa, Reproductive techniques, assisted, Sperm injections, intracytoplasmic, Fertil ization in Vitro, Pregnancy rate