Abstract: Objective：To investigate the clinical characteristics of heterotopic pregnancy （HP） after in vitro fertilization and embryo transfer （IVF-ET）. Methods：The clinical data of four patients with HP after IVF-ET was retrospectively analyzed, and then compared with literature. Results：The incidence of HP in ectopic pregnancy after IVF-ET was 15.38%. Four HP patients had tubal obstructions, did not have typical symptoms of ectopic pregnancy. They had higher levels of human chorionic gonadotrophin （β-hCG） than other patients with ectopic pregnancy after IVF-ET. Conclusions：HP is related to many factors, such as the history of surgical operation on oviducts，the number of transfer embryos. At the first stage of pregnancy, HP has the same clinical characteristics as normal uterine pregnancy.

Key words: Pregnancy, ectopic, Fertilization in vitro, Embryo transfer, Reproductive techniques, assisted, Heterotopic pregnancy