五味子乙素降低PM2.5抑制HTR细胞增殖的作用研究

doi:10.12280/gjszjk.20200605

摘要/Abstract

摘要：

目的:探索五味子乙素（Schisandrin B,Sch B）是否可以降低PM2.5对人早孕胎盘绒毛膜外滋养层（human trophoblast HTR-8/SVneo,HTR）细胞增殖的抑制作用。方法:体外实验以HTR细胞为载体,利用MTS细胞增殖实验检测不同浓度的细颗粒物（PM2.5）、Sch B单独给药以及PM2.5联合Sch B给药对HTR细胞增殖的影响。结果:10~1 000 μg/mL浓度范围内的PM2.5均抑制了HTR细胞的增殖（P<0.01）,其中150 μg/mL浓度的PM2.5抑制率达到21.97%;0.1~5 μmol/L浓度Sch B均降低了PM2.5的抑制HTR细胞增殖作用,且在0.1~2 μmol/L浓度范围内其降低PM2.5抑制HTR细胞增殖的作用随着Sch B浓度增加而增大（P<0.01）,其中2 μmol/L的Sch B对HTR细胞的保护作用最强,保护率达到14.07%。结论:Sch B可以有效降低PM2.5对HTR细胞增殖的抑制作用,具有体内潜在保护人类滋养细胞的作用。

关键词: 五味子素, PM2.5, 滋养层, 细胞增殖, 细胞培养技术

Abstract:

Objective: To investigate whether Schisandrin B (Sch B) can attenuate the effect of PM2.5 on the proliferation of human trophoblast HTR-8/Svneo (HTR) cells. Methods: The in vitro cultured HTR cells were treated by PM2.5 of 10-1 000 μg/mL concentration, Sch B-alone and Sch B plus PM2.5. The proliferation of HTR cells was tested by MTS method. Results: PM2.5 of different concentrations from 10 to 1 000 μg/mL inhibited the proliferation of HTR cells (P<0.01), and the inhibition rate of 150 μg/mL PM2.5 was 21.97%. Sch B of the concentration from 0.1 to 5 μmol/L attenuated the PM2.5-induced inhibition of HTR cells proliferation, with a dose-effect relationship among 0.1-2 μmol/L. Sch B of 2 μmol/L presented the highest protection rate (14.07%). Conclusions: Sch B could attenuate the PM2.5-induced inhibition of HTR cell proliferation, suggesting a potential protection of human trophoblast cells in vivo.

Key words: Schisandrin, PM2.5, Trophoblasts, Cell proliferation, Cell culture techniques

李晓, 郭艳丽, 韩俊婷, 邹晓萍, 董渠龙. 五味子乙素降低PM2.5抑制HTR细胞增殖的作用研究[J]. 国际生殖健康/计划生育, 2021, 40(3): 177-181.

LI Xiao, GUO Yan-li, HAN Jun-ting, ZOU Xiao-ping, DONG Qu-long. Schisandrin B Decreases the Effect of PM2.5 on HTR Cell Proliferation[J]. Journal of International Reproductive Health/Family Planning, 2021, 40(3): 177-181.

图/表 6

图1 不同浓度PM2.5对HTR细胞增殖的影响注:** 与对照组比较P<0.01。

图2 HTR细胞在各浓度PM2.5作用不同时间下的生长曲线

图3 不同浓度Sch B对HTR细胞增殖的影响注:** 与对照组比较P<0.01。

图4 进一步探索Sch B对HTR细胞增殖的影响注:* 与对照组比较P<0.05,** 对照组比较P<0.01。

图5 各浓度Sch B联合PM2.5给药对HTR细胞增殖的影响注:## 与对照组比较P<0.01;** 与PM2.5组比较P<0.01。

图6 各浓度Sch B联合PM2.5给药与PM2.5单独给药的对比注:** 与PM2.5组比较P<0.01。

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