窦前卵泡体外三维培养系统研究进展

doi:10.12280/gjszjk.20220455

摘要/Abstract

摘要：

卵泡体外培养对癌症治疗导致的卵巢功能下降患者的生育力保护有重要意义，也是研究卵泡发育和卵子发生的一种有效方法。卵泡体外培养需要建立能够较好模拟体内生长环境的体外培养系统，目前体外卵泡培养系统大致分为二维培养系统和三维培养系统。卵泡二维培养系统已在啮齿动物模型中成功建立。由于三维培养可以满足卵泡生长所需的空间结构，其培养效果优于二维培养，广泛用于各种动物模型卵泡的培养。三维培养系统可分为支架形式培养系统与无支架形式培养系统，有无支架以及支架材料的选择对卵泡体外发育有直接影响。支架材料根据来源可分为天然材料和合成材料，天然材料如海藻酸盐、胶原蛋白和纤维蛋白等在生物相容性上优于合成材料，但合成材料的可调节性使其具有良好前景。综述三维培养系统选择对卵泡体外发育的影响。

关键词: 卵泡, 卵母细胞, 细胞培养技术, 生育力, 支架, 三维细胞培养系统

Abstract:

In vitro follicle culture has important implications for preserving fertility in patients with the decreased ovarian function caused by cancer treatment. It is also an effective method for studying follicle development and oocyte maturation. In order to establish an in vitro culture system that can better simulate the in vivo growth environment, current in vitro follicle culture systems are roughly divided into two-dimensional and three-dimensional cultures. Two-dimensional follicle culture systems have been successfully established in rodent models. However, since three-dimensional culture can provide the spatial structure for follicular growth, the effect of three-dimensional culture is superior to that of two-dimensional culture. Therefore, three-dimensional culture has been widely used for the culture of follicles in various animal models. Three-dimensional culture systems can be divided into scaffold-based and scaffold-free culture systems, and the choice of scaffold material has a direct impact on in vitro follicle development. Scaffold materials can be classified into natural and synthetic materials according to their source. Natural materials, such as alginate, collagen and fibronectin, have better biocompatibility than synthetic materials, but the adjustability of synthetic materials makes them promising. This review summarizes the effects of choosing a three-dimensional culture system on in vitro follicle development.

Key words: Follicle, Oocytes, Cell culture techniques, Fertility, Stents, 3D cell culture system

李延, 胡方方, 陈欢欢, 张磊, 张翠莲, 梁琳琳. 窦前卵泡体外三维培养系统研究进展[J]. 国际生殖健康/计划生育杂志, 2023, 42(3): 221-225.

LI Yan, HU Fang-fang, CHEN Huan-huan, ZHANG Lei, ZHANG Cui-lian, LIANG Lin-lin. Research Progress of In Vitro Three-Dimensional Culture System of Preantral Follicles[J]. Journal of International Reproductive Health/Family Planning, 2023, 42(3): 221-225.

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