国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2012, Vol. 31 ›› Issue (4): 265-267.

• 论著 • 上一篇    下一篇

晚裂胚胎性染色体及18号染色体非整倍体分析

石 洁, 杨清秀, 罗海宁 ,张云山   

  1. 天津市中心妇产科医院（石 洁，罗海宁，张云山）；中国人民武装警察部队后勤医学院附属医院妇产科（杨清秀）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 张云山

Analysis on the Aneuploidy Incidence of Chromosome X/Y and 18 in Later Cleavaged Embryos

SHI Jie，YANG Qing-xiu，LUO Hai-ning，ZHANG Yun-shan   

  1. Tianjin Central Hospital of Obstetrics and Gynecology, Tianjin 300100, China（SHI Jie，LUO Hai-ning，ZHANG Yun-shan）；Department of Obstetrics and Gynecology, the Affiliated Hospital of Chinese People’s Armed Forces Logistic College, Tianjin 300162，China（YANG Qing-xiu）
  • Received:1900-01-01 Revised:1900-01-01 Published:2012-07-15 Online:2012-07-15
  • Contact: ZHANG Yun-shan

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摘要： 目的：分析晚裂胚胎性染色体及18号染色体数目异常情况。方法：应用荧光原位杂交技术检测晚裂胚胎X、Y及18号染色体的非整倍体情况，并与低评分的双原核（2PN）胚胎进行比较。结果：共收集85个胚胎，其中晚裂胚胎45个（不考虑形态学评分），成功固定269个核；低形态学评分的2PN胚胎40个，成功固定243个核，用CEPX/Y、CEP18染色体探针对85个胚胎共512个核进行荧光原位杂交。晚裂胚胎组的染色体数目异常率为45.7%，其中性染色体异常率为34.5%，18号染色体异常率为38%；2PN胚胎组的染色体异常率为48.5%，其中性染色体异常率为32%，18号染色体异常率为39%。2组性染色体和18号染色体的异常率差异均无统计学意义。结论：晚裂胚胎性染色体及18号染色体数目有较高的异常发生率，与低形态学评分的2PN胚胎一样，均不适合宫腔内移植。

关键词: 原位杂交, 荧光, 性染色体, 染色体, 人, 18对, 非整倍性, 双原核胚胎

Abstract: Objective： To analyze the aneuploidy incidence of Chromosome X，Y and 18 in later cleaved embryos. Methods：The aneuploidy of Chromosome X，Y and 18 in later cleaved embryos from MⅡoocytes and two pronuclei （2PN） zygotes was detected by the method of fluorescence in situ hybridization （FISH）. Results：There were 45 later cleaved embryos from MⅡoocytes and 40 poor scored embryos from 2PN zygotes among 85 obtained embryos. Fluorescent signals were successfully catched in 512 nuclei. The abnormality rates of X，Y and 18 chromosomes were 34.5% and 38% in later cleaved embryos，and 32% and 39% in poor scored embryos from 2PN zygotes. The differences of aneuploidy incidences of chromosome X，Y and 18 were statistically insignificant between two groups. Conclusions：There are high incidences of Chromosome X，Y and 18 in later cleaved embryos and poor scored embryos from 2PN zygotes， showing that those embryos are not suitable for embryo transfer.

Key words: In situ hybridization, fluorescence, Sex chromosomes, Chromosomes, human, pair 18, Aneuploidy, Two pronuclei zygotes