国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2018, Vol. 37 ›› Issue (5): 363-366.

• 论著 • 上一篇    下一篇

PTEN在体外受精-胚胎移植胎盘及胚胎组织中的表达及意义

赵亮，孙丽芳，郑秀丽，刘静芳，郑蓉，王颖，杨蕊，张蕾，于丽，张晗   

  1. 100035  北京积水潭医院妇产科（赵亮，孙丽芳，郑秀丽，刘静芳，郑蓉，张晗）；北京大学第三医院生殖中心（王颖，杨蕊）；北京清华长庚医院妇产科（张蕾）；北京大学第一医院生殖中心（于丽）
  • 收稿日期:2018-07-15 修回日期:2018-09-03 出版日期:2018-09-15 发布日期:2018-09-15
  • 通讯作者: 赵亮，E-mail：zhaol1972@aliyun.com E-mail:zhaol1972@aliyun.com
  • 基金资助:
    国家自然科学基金面上项目（81070493）

Expression of PTEN in the Placental and Embryonic Tissues from Patients undergoing in vitro Fertilization-Embryo Transfer

ZHAO Liang，SUN Li-fang，ZHENG Xiu-li，LIU Jing-fang，ZHENG Rong，WANG Ying，YANG Rui，ZHANG Lei，YU Li，ZHANG Han   

  1. Reproductive Medical Center，Peking University Third Hospital，Beijing 100191，China（WANG Ying，YANG Rui）；Department of Obstetrics and Gynecology，Beijing Tsinghua Chang Gung Hospital，Beijing 102218，China（ZHANG Lei）；Reproductive Medical Center，Peking University First Hospital，Beijing 100034，China（YU Li）
  • Received:2018-07-15 Revised:2018-09-03 Published:2018-09-15 Online:2018-09-15
  • Contact: ZHAO Liang，E-mail：zhaol1972@aliyun.com E-mail:zhaol1972@aliyun.com

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摘要： 目的：研究体外受精-胚胎移植（IVF-ET）对早期胎盘绒毛和胚胎组织中第10号染色体上缺失的磷酸和张力蛋白同源基因（PTEN）和蛋白表达的影响。方法：收集8例IVF-ET患者胚胎移植后30～45 d行减胎术的胎盘绒毛组织和胚胎组织作为IVF-ET组，10例自然妊娠行人工流产患者的早期胎盘绒毛组织和胚胎组织为对照组。采用免疫组织化学方法和实时荧光定量聚合酶链反应（qRT-PCR）方法检测组织中PTEN蛋白和mRNA的表达及分布差异。结果：PTEN蛋白表达于胎盘绒毛细胞滋养层细胞和合体滋养层细胞的细胞膜和细胞质，广泛表达于胚胎各个胚层。IVF-ET组胎盘绒毛组织中PTEN蛋白表达量低于对照组，差异有统计学意义（t=2.325，P=0.027）；PTEN蛋白在2组患者胚胎组织中表达量差异无统计学意义（P=0.288）。PTEN mRNA在IVF-ET组胎盘绒毛组织中表达量低于对照组，差异有统计学意义（t=2.512，P=0.017），在2组患者胚胎组织中表达量比较，差异无统计学意义（P=0.371），与免疫组织化学结果一致。结论：IVF-ET技术的体外操作可能影响胎盘组织PTEN基因和蛋白的表达，但未显著影响早期胚胎组织的PTEN表达，其潜在意义有待进一步研究。

关键词: 受精, 体外, 胚胎移植, 胎盘, PTEN, 胚胎

Abstract: Objective：To study the effect of in vitro fertilization-embryo transfer (IVF-ET) on the expression of the gene of phosphate and tension homology deleted on chromsome ten (PTEN) and its protein in the placenta villus and embryonic tissues. Methods：Eight cases undergoing IVF-ET and the ultrasound-guided embryo reduction (the IVF-ET group), and 10 cases of natural pregnancy undergoing abortion (the control group) were included in this study. The placental villus and embryonic tissue were collected. The expression of PTEN mRNA was measured by real-time qPCR. The distribution of PTEN protein in tissues was tested by immunohistochemistry. Results：PTEN protein was expressed in the cell membrane and cytoplasm of placental villus trophoblast cells and syncytiotrophoblast cells, and widely expressed in the different germ layers of early embryo. The expression of PTEN protein in the placental villus of the IVF-ET group was lower than that of the control group (t=2.325, P=0.027). However, there was no significant difference in the expression of PTEN protein in the tissues of early embryo between the two groups (P=0.288). The expression pattern of PTEN mRNA was just consistent with that of PTEN protein. Conclusions：The in vitro procedure of IVF-ET may decrease the expressions of PTEN gene and protein in placenta, however does not change the expression levels in the tissues of early embryo. It is necessary to explore the underlying meanings of the decreased PTEN expression in placenta related to IVF-ET.

Key words: Fertilization in vitro, Embryo transfer, Placenta, PTEN, Embryo