国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2011, Vol. 30 ›› Issue (1): 42-44.

• 论著 • 上一篇    下一篇

IGF-Ⅱ、EGF对ICR小鼠早期胚胎体外发育的影响

潘 景 ,刘 洋, 周红林   

  1. 650101 昆明医学院第二附属医院妇科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 周红林

Effect of IGF-Ⅱ and EGF on the Development of ICR Mouse Embryos in Vitro

PAN Jing, LIU Yang, ZHOU Hong-lin   

  1. Department of Gynecology, the Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China
  • Received:1900-01-01 Revised:1900-01-01 Published:2011-01-15 Online:2011-01-15
  • Contact: ZHOU Hong-lin

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摘要： 目的：研究胰岛素样生长因子Ⅱ（IGF鄄Ⅱ）、表皮生长因子（EGF）在含葡萄糖、胰岛素的CZB
培养液中对ICR 小鼠早期胚胎体外发育的影响。方法：①在含有葡萄糖、胰岛素的CZB培养液中分别添
加0，0.1，1，10，100 μg/L 的IGF鄄Ⅱ，体外培养观察其对ICR 小鼠1-细胞胚胎的作用，并设单独CZB培养
液对照组。②在含葡萄糖、胰岛素和0.1 μg/L IGF鄄Ⅱ的CZB 培养液中分别添加0，0.1，1，10，100 μg/L
EGF，体外培养观察其对ICR 小鼠1- 细胞胚胎的作用，并设单独CZB 培养液对照组。比较各组囊胚率、孵
化率和胚胎细胞数的变化。结果：① 0 μg/L IGF鄄Ⅱ组囊胚率、孵化率及囊胚细胞数高于对照组（P＜0.05）。
100 μg/L IGF鄄Ⅱ组囊胚率、孵化率及囊胚细胞数低于0，0.1，1，10 μg/L IGF鄄Ⅱ各组（P＜0.05）；0.1 μg/L
IGF鄄Ⅱ组囊胚细胞数最高（P＜0.05）。② 0 μg/L EGF组囊胚率、孵化率及囊胚细胞数高于对照组（P＜
0.05）。0.1，1，10μg/L EGF 各组囊胚率、孵化率高于0，100 μg/L EGF 组（P＜0.01）；0.1 μg/L EGF组孵化
率最高（P＜0.05），囊胚细胞数高于0μg/L EGF 组（P＜0.01）；100μg/L EGF组囊胚细胞数最低（P＜0.05）。
结论：① 1，10μg/L IGF鄄Ⅱ和葡萄糖及胰岛素相互作用对胚胎细胞无明显抑制或促进作用。② 1，10 μg/L
EGF 参与葡萄糖、胰岛素、IGF鄄Ⅱ对胚胎细胞分化的调节，却无增殖作用。③0.1 μg/L IGF鄄Ⅱ和EGF 作为
胚胎培养浓度较佳。④100μg/L IGF鄄Ⅱ和EGF 对胚胎有抑制作用。

关键词: 胰岛素样生长因子Ⅱ, 表皮生长因子, 小鼠, 胚胎发育, 体外研究

Abstract: Objective: To investigate the effection of IGF-域and EGF on the development of ICR mouse
preimplantation embryos in the CZB culture medium with glucose and insulin in vitro. Methods: 淤Experiment1:
One-cell ICR mouse embryos were cultured in the CZB medium with glucose and insulin supplemented with IGF-
域（0, 0.1, 1, 10 and 100 滋g/L）, and a separate control group of CZB medium was established .于Experiment 2:
One-cell ICR mouse embryos were cultured in the CZB medium with glucose, insulin and IGF-域（0.1 滋g/L）
supplemented with EGF （0, 0.1, 1, 10 and 100 滋g/L）, and a separate control group of CZB medium was
established. Blastocyst rates, hatched blastocyst rates and the cell numbers of blastocysts were counted. Results:
①Experiment1: IGF-Ⅱ（0 μg/L）increased the rate of blastocyst, hatched blastocyst and the total cell numbers of
blastocyst compared with control group（P＜0.05）. IGF-Ⅱat a dose of 100 μg/L decreased the rate of blastocyst,
hatched blastocyst and the total cell numbers of blastocyst compared with the other groups（P＜0.05）. IGF-Ⅱ at a
dose of 0.1 μg/L greatly increased the total cell numbers of blastocyst（P＜0.05）. ②Experiment 2: EGF（0 μg/L）
increased the rate of blastocyst, hatched blastocyst and the total cell numbers of blastocyst compared with control
group（P＜0.05）. EGF at doses of 0.1, 1 and 10 μg/L increased the rate of blastocyst and hatched blastocyst
compared with EGF at doses of 0 and 100 μg/L（P＜0.05），and EGF at a dose of 0.1 μg/L had the highest rate of
hatched blastocyst（P＜0.05）. EGF at a dose of 0.1 μg/L increased the total cell numbers of blastocyst compared
with EGF at a dose of 0μg/L（P＜0.05）. EGF at a dose of 100 μg/L had the lowest rate of the total cell numbers
of blastocyst（P＜0.05）. Conclusions:淤IGF-域渊1, 10 滋g/L冤, glucose and insulin showed no significant effect of
suppression or promotion among. 于EGF 渊1, 10 滋g/L冤in CZB medium with glucose, insulin and IGF-域could
involves in the regulationof differentiation of embryonic cells but no proliferation. 盂IGF-域and EGF at a dose of
0.1 滋g/L is best for cell culture concentration. 榆IGF-域and EGF at a dose of 100 滋g/L have inhibition effect on
embryonic cells.

Key words: Insulin鄄like growth factor II, Epidermal growth factor, Mice, Embryonic development, In vitro