国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2011, Vol. 30 ›› Issue (1): 39-41.

• 论著 • 上一篇    下一篇

含雌激素的冷冻保护剂对冻存正常精子质量的影响

李凤华，赵君利，贾韶彤，王红燕   

  1. 750004 银川，宁夏医科大学（李凤华，王红燕）；宁夏 医科大学附属医院生殖医学中心（赵君利，贾韶彤）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 赵君利

Effects of the Cryoprotectants Containing Estrogen on the Sperm Vitrified Cryopreservation

LI Feng-hua，ZHAO Jun-li，JIA Shao-tong，WANG Hong-yan   

  1. Ningxia Medical University，Yinchuan 750004, China （LI Feng鄄hua，WANG Hong鄄yan）；Reproductive Center of the Affiliated Hospital of Ningxia Medical University，Yinchuan 750004, China（ZHAO Jun鄄li，JIA Shao鄄tong）
  • Received:1900-01-01 Revised:1900-01-01 Published:2011-01-15 Online:2011-01-15
  • Contact: ZHAO Jun-li,

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摘要： 目的：探讨含雌激素的冷冻保护剂对正常精液精子玻璃化冻存效果的影响。方法：将门
诊收集的每例正常健康人精液平均分成5 份，1 份用于冷冻前指标测定，其余4 份分别应用冷冻保护剂
（CPM）甘油、甘油- 卵黄- 柠檬酸钠（GYC）、甘油+ 雌二醇（E2）、GYC+E2进行玻璃化冻存，检测冻存前、
后精子活率、活力（a+b 级）、正常精子形态百分率和精子尾部低渗肿胀率（HOSR）等精子功能的指标，评
价4种CPM 的冷冻保护效果。结果：4 种CPM对精子的冷冻保护效果有区别，精子活率和活力、正常形态
率和HOSR在冷冻后都有不同程度下降（P＜0.01），甘油组、GYC 组比甘油+E2 组和GYC+E2组下降更明
显（P＜0.01）。GYC+E2组各参数高于甘油+E2组。结论：精子冷冻保护剂中加入雌激素可减少对正常精液
冻融过程中精子的损伤，含雌激素的甘油复合型冷冻保护剂冷冻保护效果优于含雌激素的甘油型冷冻
保护剂。

关键词: 精子, 精液保存, 雌激素类, 冷冻, 甘油, 雌二醇, 精子能动性, 精子尾

Abstract: Objective: To explore the different effect of different cryoprotective containing estrogen on
human spermatozoa. Methods: Each semen came from healthy patient will be divided into five copies, one
copy was as control group, and the other four copies were as experimental group which were preserved by
cryopreservativemedium泽（悦PMs）with glycerol, glycerol鄄eygyolk鄄sodium（GYC）, glycerol+estrogen and GYC+
estrogen. The cryoprotective effects of human spermatozoa were evaluated by conventional semen analysis,
viability, motility, morphology and spermatozoa hypo鄄osmotic swelling rate （HOSR）. Results: During the
procedure of cryoprotective and thawing, different CPM had different protective effect on the spermatozoa,
which showed significantly different（P<0.01）, and glycerol and glycerol鄄eygyolk鄄sodium（GYC）group were
decreased significantly than the other two groups（P<0.01）. The parameters in the the group of GYC+E2 were
significantly higher than those of in the group of glycerol +E2 （P<0.01）. Conclusions: The cryoprotectant
containing estrogen can increase the function of cryopreserved sperm.

Key words: Spermatozoa, Semen preservation, Estrogens, Freezing, Glycerol, Estradiol, Sperm motility, Sperm tail