国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (5): 348-350.

• 论著 • 上一篇    下一篇

D3形态学低评分胚胎囊胚形成的影响因素分析

李云，全松，史潇，罗琛   

  1. 510515 南方医科大学（李云）；南方医科大学南方医院妇产科生殖中心（全松，史潇，罗琛）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-09-15 发布日期:2014-09-15
  • 通讯作者: 全松

Analysis of Factors Influencing Blastocyst Formation of the Embryos with Low Score on Day 3

LI Yun，QUAN Song，SHI Xiao，LUO Chen   

  1. Southern Medical University, Guangzhou 510515, China（LI Yun）；Center of Reproductive Medicine，Department of Obstetrics and Gynecology，Nanfang Hospital，Southern Medical University， Guangzhou 510515，China（QUAN Song, SHI Xiao, LUO Chen）
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-09-15 Online:2014-09-15
  • Contact: QUAN Song

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摘要： 目的：探讨取卵后第3天（D3）形态学低评分胚胎体外囊胚培养的发育潜能，分析影响囊胚形成的临床及胚胎因素。方法：通过序贯培养将D3低评分胚胎继续培养至囊胚期，观察囊胚形成率及优质囊胚形成率。分析年龄、基础窦卵泡数、基础卵泡刺激素（bFSH）、促性腺激素（Gn）总剂量、人绒毛膜促性腺激素（hCG）日雌二醇（E2）水平、获卵数、D3卵裂细胞数、D3胚胎碎片与囊胚形成的关系。结果：共收集422个取卵周期1 745个低评分胚胎进行囊胚培养，共形成1 046个（59.9%）囊胚，763个（43.7%）优质囊胚。不同基础窦卵泡、bFSH、Gn总剂量、hCG日E2水平、获卵数组间囊胚形成率及优质囊胚形成率差异均无统计学意义（P＞0.05）；不同年龄组间囊胚形成率差异无统计学意义（P＞0.05），但优质囊胚形成率差异有统计学意义（P＜0.05）；不同D3卵裂细胞数、D3胚胎碎片组间囊胚形成率及优质囊胚形成率差异均有统计学意义（P＜0.05）。结论：低评分胚胎有不同程度的发育潜能，D3卵裂细胞数、D3胚胎碎片影响低评分胚胎的囊胚形成；年龄、D3卵裂细胞数、D3胚胎碎片影响低评分胚胎优质囊胚的形成。

关键词: 胚胎发育, 囊胚, 因素分析, 统计学, 细胞, 培养的

Abstract: Objective: To explore the developmental potentiality of the embryos with low morphological score on Day 3(D3) during in vitro blastocyst culture, and analyze the clinical and embryonic factors affecting the blastocyst formation. Methods：Those embryos with D3 low score were further cultured to form blastula through the sequential methods. The blastocyst formation rate and the high-quality blastocyst formation rate were calculated. Those factors related to blastocyst formation were analyzed, including age，antral follicle count，bFSH， total dose of Gn，E2 level on hCG day，the number of retrieved oocytes，the number of blastomere on D3, and the embryonic fragmentation on D3. Results：A total of 1 745 low score embryos from 422 cycles were collected. 1 046 blastulas were formed（59.9%） after in vitro culture，among which 763（43.7%） were high-quality blastulas. There were no significant differences in the blastocyst formation rate and the high-quality blastocyst formation rate among subgroups grouping with the antral follicle count，bFSH， total dose of Gn，E2 levels on hCG day, or the number of retrieved oocytes（P＞0.05）. There was no significant difference in the blastocyst formation rates among subgroups grouping with age（P＞0.05），while there was significant difference in the high-quality blastocyst formation rate among those subgroups （P＜0.05）. Particularly, there were significant differences in the blastocyst formation rate and the high-quality blastocyst formation rate among subgroups grouping with the number of blastomere or the degree of embryonic fragmentation on D3（P＜0.05）. Conclusions：Embryos with low score have developmental potentiality on different degrees. The number of blastomere and the degree of embryonic fragmentation on D3 significantly influence the blastocyst formation of those embryos with low score. The factors of age, the number of blastomere and the degree of embryonic fragmentation significantly influence the high-quality blastocyst formation of embryos with low score.

Key words: Embryonic development, Blastula, Factor analysis, statistical, Cells, cultured