国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2015, Vol. 34 ›› Issue (3): 181-183.

• 论著 •    下一篇

五味子乙素对苯并芘致HTR8-SVneo细胞氧化损伤的保护作用

章艳燕，侯海燕，陈晓，陈亚琼   

  1. 300162 天津，中国人民武装警察部队后勤学院附属医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-05-15 发布日期:2015-05-15
  • 通讯作者: 陈亚琼

Protective Effect of Schizandrin B on Oxidative Damage Induced by BaP in HTR8-SVneo Cells

ZHANG Yan-yan，HOU Hai-yan，CHEN Xiao，CHEN Ya-qiong   

  1. Department of Obstetrics and Gynecology, Affiliated Hospital of Logistics University of Chinese People′s Armed Forces，Tianjin 300162，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-05-15 Online:2015-05-15
  • Contact: CHEN Ya-qiong

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摘要： 目的：研究环境污染物苯并芘（BaP）致人绒毛膜滋养层细胞HTR8-SVneo氧化损伤的作用机制，探讨五味子乙素（Sch B）的可能保护作用。方法：本实验分为空白组、BaP组、Sch B不同浓度组（0.1，0.5，2.0 μmol/L），以HTR8-SVneo 细胞为载体，构建BaP氧化应激损伤模型，测定氧化和抗氧化指标。结果：与空白组比较，BaP组超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的浓度显著降低，而乳酸脱氢酶（LDH）、丙二醛（MDA）、一氧化氮（NO）和诱导型一氧化氮合酶（iNOS）的浓度明显增加（P＜0.05）。不同剂量Sch B组SOD、GSH-Px的浓度明显提高，LDH、MDA、NO、iNOS的浓度减少，与BaP组比较，差异有统计学意义（P＜0.05）。结论：Sch B能调节BaP所致的HTR8-SVneo细胞氧化系统和抗氧化系统的失衡，从而预防BaP致HTR8-SV neo细胞的氧化损伤。

关键词: 苯并芘, 五味子素, 滋养层, 超氧化物歧化酶, 谷胱甘肽过氧化酶, L-乳酸脱氢酶, 丙二醛, 一氧化氮, 一氧化氮合酶

Abstract: Objective: To study the oxidative damage of benzo[a]pyrene（BaP） in HTR8-SVneo cells, and to explore the protective effect of Schizandrin B（Sch B）. Methods：The HTR8-SVneo cells were in vitro cultured, and the oxidative stress damage model was induced by BaP of different doses （0.1，0.5，2.0 μmol/L）. The experiment were designed as the control group, the BaP group, and the Sch B（0.1，0.5，2.0 μmol/L） groups. The oxydic parameters and antioxidative indexes were detected. Results：Compared with control group，the BaP group had significantly lower levels of GSH-Px and SOD and higher levels of LDH，MDA，NO and iNOS（P＜0.05）. The Sch B groups（0.1,0.5,2.0 μmol/L） had significantly higher levels of SOD and GSH-Px and lower levels of LDH，MDA，NO and iNOS than the BaP group（P＜0.05）. Conclusions：Sch B can rebalance between oxidation and anti-oxidation in the HTR8-SVneo cells treated by BaP， suggesting the protective effects of Sch B on oxidative damage.

Key words: Benzo（a）pyrene, Schizandrin, Trophoblasts, Superoxide dismutase, Glutathione peroxidase, L-lactate dehydrogenase, Malondialdehyde, Nitric oxide, Nitric oxide synthase