国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2015, Vol. 34 ›› Issue (3): 184-188.

• 论著 • 上一篇    下一篇

双酚A对诱导型一氧化氮合酶基因缺失鼠卵母细胞染色体不分离的影响

张金文，杜春海，张杰   

  1. 050000 石家庄，河北医科大学第二医院血管外科（张金文）；河北省景县人民医院血管外科（杜春海）；河北医科大学第四医院妇瘤科（张杰）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-05-15 发布日期:2015-05-15
  • 通讯作者: 张金文

Effect of Bisphenol A on the Oocyte Chromosome Non-disjunction in iNos-/- Mice

ZHANG Jin-wen，DU Chun-hai，ZHANG Jie   

  1. Department of Vascular Surgery，The Second Hospital of Hebei Medical University，Shijiazhuang 050000，China（ZHANG Jin-wen）；Department of Vascular Surgery，The Jingxian Renmin Hospital，Jingxian 053500，Hebei Province，China（DU Chun-hai）；Department of Gynecologic Oncology,The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China（ZHANG Jie）
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-05-15 Online:2015-05-15
  • Contact: ZHANG Jin-wen

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摘要： 目的：研究双酚Ａ（ｂｉｓｐｈｅｎｏｌ Ａ，ＢＰＡ）暴露对诱导型一氧化氮合酶（ｉｎｄｕｃｉｂｌｅ ｎｉｔｒｉｃ ｏｘｉｄｅ ｓｙｎｔｈａｓｅ，ｉＮos）基因缺失（ｉＮos－／－）的实验鼠卵母细胞染色体不分离的影响。方法：给野生型雌鼠（ｉNos+/+）和iNos基因敲除（iNos-/-）的雌鼠强饲（oral gavage） BPA 100 μg/（kg·d）或200 μg/（kg·d）连续13 d。结果：在iNos+/+组实验鼠卵细胞MⅡ期非整倍体卵母细胞数量没有显著增加。在iNos-/-组实验鼠卵母细胞染色体分离错误和染色单体提前分离增加。基因表达的特性表明polo样激酶1（PLK1）和RAN GTPase蛋白（RAN）水平在iNos-/-组实验鼠卵细胞的细胞周期和纺锤体的调控大幅下降。结论：iNos可能通过维持蛋白PLK1和蛋白RAN在哺乳类动物卵细胞稳定地表达，直接或间接地保护染色体减数分裂中精确分离；低水平的BPA暴露可能引起具有iNos-/-遗传背景卵细胞染色体畸变。

关键词: 一氧化氮合酶, 减数分裂, 非整倍性, 卵母细胞, 有丝分裂纺锤体, 双酚A

Abstract: Objective: To explore the effects of bisphenol A （bisphenol A, BPA） on the oocyte chromosome non-disjunction in iNos-/- mice. Methods：Female iNos+/+ mice and iNos-/- mice, aged 20-22 days, were treated with BPA （100 or 200 μg/kg body weight per day） by intragastric administration for 13 days. The isopyknic corn oil was used as control. Results：In the iNos+/+ mice groups, the numbers of aneuploidy oocytes at metaphase MⅡ were not increased by BPA treatment. Interestingly, the numbers of those oocytes with the error-prone chromosome segregation and the precocious chromatid separation in the iNos-/- mice groups were significantly increased by BPA treatment. Meanwhile, the expressions of polo-like kinase-1（PLK1） and RAN GTPase（RAN） in oocytes of the iNos-/- mice were significantly down-regulated by BPA treatment. Conclusions：iNos has a direct or indirect protection against the faithful chromosome segregation during meiosis, possibly by maintaining stable expression of PLK1 and RAN in mammalian oocytes. BPA exposure at low level may induce chromosomal aberrations in those oocytes of iNos-/- mice.

Key words: Nitric oxide synthase, Meiosis, Aneuploidy, Oocytes, Mitotic spindle apparatus, Bisphenol A