Abstract: Objective： To evaluate the application value of HR-HPV and colposcopy in CIN and invasive cervical cancer diagnosis，when ASCUS was diagnosed by the cervical cytology，and to build the clinical diagnose pathway. Methods：320 patients who were diagnosed as ASCUS underwent the colposcopy and HR-HPV test. The relationship between ASCUS and patients' age was also analysed. Results：In ASCUS patients，49.38% patients were aged from 30 to 49 years. The histo-pathological results of 320 ASCUS were as follows：inflammation 73.13% （234/320），condyloma 5.32% （17/320），CIN （included CINⅠ，CINⅡ，CINⅢ） 20.61% （66/320），high grade CIN（included CINⅡ，CINⅢ） and invasive cancer 7.5%（24/320）. 144 patients were HR-HPV positive，and the positive rate was 45%（144/320）. In HR-HPV positive group，43.75% patients （63/144） were CIN. In HR-HPV negative group，only 3.41% patients（6/176）were CIN. Ｃｏｎｃｌｕｓｉｏｎs： Those ASCUS patients with HR-HPV positive，especially between 30-year to 49-year，should undergo colposcopy. Those patients with HR-HPV negative might not undergo colposcopy，but they should receive the TCT and HR-HPV test regularly.

Key words: Human papillomavirus, Colposcopy, Cervical intraepithelial neoplasia, Atypical squamous cells of undetermined significance