Abstract: Objective：To investigate the predictive value of serum β-hCG and progesterone （P4） for the early pregnancy outcome on the fourteenth day after in vitro fertilization and embryo transfer （IVF-ET）. Methods：The levels of serum β-HCG and P4 of 554 patients treated with IVF/introcytoplasm sperm injection （ICSI） in our center from April 2010 to November 2011 were analyzed retrospectively. Patients were divided into the normal pregnancy group，the abnormal pregnancy group and the non-pregnant group. The normal pregnancy included single pregnancy and multiple pregnancy. The abnormal pregnancy included early pregnancy miscarriage，ectopic pregnancy and biochemical pregnancy. The differences of β-hCG and P4 among groups were compared. Results：①The level of serum β-hCG in the normal pregnancy group was higher than those in the early abortion/ectopic pregnancy group，the biochemical pregnancy group and non-pregnant group（P＜0.05）. There was no statistical differences of β-hCG between the early abortion group and ectopic pregnancy group（P＞0.05）. ②The level of serum P4 in the normal pregnancy group was higher than those in the abnormal pregnancy group and the non-pregnant group （P＜0.05）, while the level in the multiple pregnancy group was significantly higher than that in the singleton pregnancy group （P＜0.05）. ③When the level of β-hCG of single test was＞197.27 IU/L, the possibility of normal pregnancy was 88.9%. When the level of β-hCG of single test was＞613.94 IU/L，there were a better pregnancy outcome and a higher possibility of multiple pregnancy. Conclusions：Serum β-hCG and progesterone on the fourteenth day after transplantation can be used as the good combined markers of early pregnancy outcome of IVF-ET.

Key words: Fertilization in vitro, Embryo transfer, Pregnancy outcome, Chorionic gonadotropin, Progestins