Journal of International Reproductive Health/Family Planning

Journal of International Reproductive Health/Family Planning ›› 2015, Vol. 34 ›› Issue (5): 361-363.

• 论著 • Previous Articles     Next Articles

Protective Effect of Astragalus Polyose on DNA Integrity of Human Sperm

LIU Li-jun，MA Peng-cheng，WANG Lei，ZHANG Ai-ping，WANG Yan-chao，WANG Jing   

  1. Human Sperm Bank，The First Hospital of Lanzhou University，Lanzhou 730000，China（LIU Li-jun，MA Peng-cheng，ZHANG Ai-ping，WANG Yan-chao）；Institute of Developmental Biology，School of Life Science, Lanzhou University，Lanzhou 730000，China（WANG Lei）；School of Medical Examination，Gansu University of Traditional Chinese Medicine，Lanzhou 730000，China（WANG Jing）
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-09-15 Online:2015-09-15
  • Contact: WANG Jing

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Abstract: Objective: To examine the protective effect of astragalus polyose (AP) on the DNA integrity of human sperm underwent freezing-thawing. Methods: The human semen samples with normal motility (7 samples) or low motility (5 samples) were collected. After grouped, the sperm suspension were freezed with the cryoprotective medium contained 0, 10, 25 and 50 mg/L of AP (the T0, T1, T2, T3 groups). The positive control is 1 g/L of Vitamin E (the T4 group). After the conventionally freezing-thawing, and dyeing with acridine orange, those sperm samples were detected the DNA fragmentation index (DFI) using fluorescence microscope. Results: In those groups of normal sperm motility, the DFI in the T1 group was significantly lower than those in the T0, T2, T3 and T4 groups (P＜0.05). In those groups of low sperm motility, the DFI in the T1 group was significantly lower than those in the T0 group and the T4 group (P＜0.05). Conclusions: The AP at 10 mg/L concentration has a protective effect on the DNA integrity of human sperm underwent freezing-thawing.

Key words: Astragalan, Semen preservation, Cryoprotective agents, DNA Fragmentation