Abstract: Follicular fluid is a biological fluid containing a varity of the proteins and factors secreted by oocytes, granulosa cells and theca cells, which is important for the follicular development and oocyte maturation as the medium or micro-environment of oocyte growth. Over the past ten years, the proteomic studies of follicular fluid were focused on the identification of some novel proteins and screening of disease-related proteins. In this article, five aspects related to the proteomic studies of follicular fluid were reviewed as follows: some new proteins identified from follicular fluid, the age-related biomarkers, some definite biomarkers of follicular maturation, the disease-related markers, and the biomarkers related to IVF success. It is possible that the quality of follicle and oocyte, and the potential of fertilization and embryo development, be predicted by some potential biomarkers of follicular fluid that are the key reproduction-related and（or） developemnt-related proteins screened and identified from those proteomic studies.

Key words: Follicular fluid, Proteomics, Biological markers, Fertilization in vitro