Abstract: Objective：To analyze the karyotype in those couples who had one or more aneuploidy children. Methods：The G-banding was used to analyze the chromosome karyotype in 273 couples with the history of aneuploidy children (the test group) and 116 normal couples (the control group). The C-banding was also used if necessary. Results：In the test group, there were 72 chromosomal abnormalities including 4 structural abnormalities(the detection rate 1.46%), and 68 chromosome polymorphisms (the detection rate 24.91%). The detection rate of chromosome polymorphisms  was 25.10% (64/255) if excluding those samples from ageing women. In the control group, only one structural abnormality was found, and 5 chromosome polymorphisms were found (the detection rate 4.31%). There was significant difference in the detection rate of chromosome polymorphisms between the two groups (P＜0.05). Conclusions：There was a higher rate of chromosomal polymorphisms in those couples with the history of a or more aneuploidy children, suggesting us to pay more attention to the karyotype of those couples.

Key words: Aneuploidy, Polymorphism, genetic, Chromosome banding, Chromosome aberrations, Karyotyping