Abstract: Cell is the fundamental unit of the structure and function of lives. The cell fate is a process that decided at the single-cell level. Due to genomic and epigenetic reprogramming, as well as errors in cell division and differentiation, cells from the same lineage or individual varies in genomes, transcriptomes and epigenomes, which is the so-called heterogeneity. Single cell sequencing allows the high-throughput molecular detection of individual cell, which not only has a powerful function in solving biological heterogeneity, but also has many advantages in rare cells problems. The single-cell transcriptome sequencing (scRNA-seq) plays an important role in the observation of mammalian gamete development and early embryo development, and is of great significance for the reproductive development. In this review, we discuss recent progress in methodology and applications of the scRNA-seq.

Key words: RNA, Transcription, genetic, Genes, Sequence analysis, RNA, Germ cells