Abstract: Objective: To screen the differentially-expressed proteins in sperm of patients with asthenozoospermia, to perform bioinformatics analysis, and to elucidate the important biological processes, molecular functions and related metabolic pathways, so as to provide new ideas for further mechanism research. Methods： The sperm samples were collected from 30 normal men (as the control group) and 30 asthenospermia patients, respectively. The differentially-expressed proteins in the asthenospermia group were screened and identified by tandem mass spectrometry tag technology. Bioinformatics analysis was performed by GO and KEGG. Results：With P＜0.05 and the expression ratio ≥1.2 or ≤0.833 as the standard, there were 1 020 differently-expressed proteins, including 606 upregulated proteins and 414 downregulated proteins. GO analysis showed that the differentially-expressed proteins were mainly involved in the mRNA decomposition process and the terminating translation process. They were distributed in ribosomes and cytoplasm, with binding function and receptor-activition function. The KEGG signaling pathway analysis showed that the differentially-expressed proteins were involved in 257 pathways including metabolic pathway, adenylate activated protein kinase (AMPK) signaling pathway and oxidative phosphorylation pathway. Conclusions：The Differentially-expressed proteins in patients with asthenozoospermia were involved in complex biological processes, functions and pathways. This study may provide biological information for further research on the pathogenesis of asthenozoospermia.

Key words: Asthenozoospermia；, Spermatozoa；, Proteomics；, Signal transduction；, Computational biology