Bioinformatics Analysis to Identify Oxidative Stress-Related Key Genes in Teratozoospermia and Predict Candidate Traditional Chinese Herbal Medicines

doi:10.12280/gjszjk.20250163

Abstract

Abstract:

Objective: To identify the oxidative stress-related key genes that differentially expressed (OS-DEG) in teratozoospermia through bioinformatics analysis, and to predict the candidate targeted Chinese herbal medicines. Methods: The GSE6969 gene expression profile from the GEO (Gene Expression Omnibus) database was retrieved, and the differentially expressed genes were screened. These genes were then intersected with the oxidative stress-related genes that retrieved from the GeneCards database. The key pathways and genes were identified through Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and protein-protein interaction (PPI) network analysis. Finally, the potential candidate herbal medicines targeting those key genes were searched from the Encyclopedia of Traditional Chinese Medicine (ETCM) database. Results: A total of 261 OS-DEGs Key genes were identified by differential analysis, including PTEN, JUN, glycogen synthase kinase 3β (GSK3β), and superoxide dismutase 2 (SOD2) were screened using Cytoscape. The candidate Chinese herbal medicines were identified, such as Portulaca oleracea, Glehnia littoralis, Pinellia ternata, Verbena officinalis, Salvia miltiorrhiza, Typhonium giganteum. Conclusions: In this study, bioinformatics analysis was used to screen the genes related to oxidative stress in teratozoospermia, which provided a new insight into the treatment of teratozoospermia with traditional Chinese medicine.

Key words: Teratozoospermia, Oxidative stress, Genes, Drugs, Chinese herbal, Bioinformatics analysis

DU Chao, HOU Kai-bo, GUAN Xiao-chuan, WANG Bo-lun, WU Ying, YU Yue-xin. Bioinformatics Analysis to Identify Oxidative Stress-Related Key Genes in Teratozoospermia and Predict Candidate Traditional Chinese Herbal Medicines[J]. Journal of International Reproductive Health/Family Planning, 2025, 44(5): 361-365.

Figures/Tables 4

References 23

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排名	CLOSENESS	DEGREE	MNC	MCC
1	PTEN	JUN	JUN	PTEN
2	JUN	PTEN	PTEN	GSK3β
3	GSK3β	GSK3β	GSK3β	HNRNPA1
4	SOD2	NPM1	NPM1	PABPC1
5	SNCA	BRCA1	SOD2	YBX1
6	NPM1	SOD2	BRCA1	G3BP1
7	SDHA	SDHA	TARDBP	HNRNPA2B1
8	PARK7	TARDBP	SNCA	HNRNPD
9	MAPK1	SNCA	SDHA	FUS
10	BRCA1	MAPK1	HNRNPA1	FXR1

排名	CLOSENESS	DEGREE	MNC	MCC
1	PTEN	JUN	JUN	PTEN
2	JUN	PTEN	PTEN	GSK3β
3	GSK3β	GSK3β	GSK3β	HNRNPA1
4	SOD2	NPM1	NPM1	PABPC1
5	SNCA	BRCA1	SOD2	YBX1
6	NPM1	SOD2	BRCA1	G3BP1
7	SDHA	SDHA	TARDBP	HNRNPA2B1
8	PARK7	TARDBP	SNCA	HNRNPD
9	MAPK1	SNCA	SDHA	FUS
10	BRCA1	MAPK1	HNRNPA1	FXR1

序号	基因	中药材
1	SOD2	马齿苋、槟榔、白附子、地黄、苍耳子、山药、板蓝根、白术、山茱萸、桑叶、天冬
2	PTEN	紫苏子、北沙参
3	JUN	半夏、旋覆花、麻黄、钩藤、枳壳、附子
4	GSK3β	薤白、山慈菇、马鞭草、地黄、平贝母、麦冬、苍术、山药、灵芝、大蒜、板蓝根、枸骨叶、北沙参、枸杞子、白芷、浙贝母、人参、土贝母、大青叶、川贝母
5	SDHA	丹参、百部、白附子、山楂、蒲黄、芦荟、苍耳子、牵牛子、片头七、刺五加、两面针、板蓝根、天麻、石草、中节风、蓖麻子、明党参、罗布麻叶、肉苁蓉、党参、苦楝皮、苦参、北沙参、木贼、紫苏叶、银柴胡、半边莲、人参、紫草、紫花地丁、巴戟天、芦根、荆芥、绵萆薢、当归
6	MAPK1	马齿苋、合欢皮

序号	基因	中药材
1	SOD2	马齿苋、槟榔、白附子、地黄、苍耳子、山药、板蓝根、白术、山茱萸、桑叶、天冬
2	PTEN	紫苏子、北沙参
3	JUN	半夏、旋覆花、麻黄、钩藤、枳壳、附子
4	GSK3β	薤白、山慈菇、马鞭草、地黄、平贝母、麦冬、苍术、山药、灵芝、大蒜、板蓝根、枸骨叶、北沙参、枸杞子、白芷、浙贝母、人参、土贝母、大青叶、川贝母
5	SDHA	丹参、百部、白附子、山楂、蒲黄、芦荟、苍耳子、牵牛子、片头七、刺五加、两面针、板蓝根、天麻、石草、中节风、蓖麻子、明党参、罗布麻叶、肉苁蓉、党参、苦楝皮、苦参、北沙参、木贼、紫苏叶、银柴胡、半边莲、人参、紫草、紫花地丁、巴戟天、芦根、荆芥、绵萆薢、当归
6	MAPK1	马齿苋、合欢皮