Abstract: Objective：To observe the expression changes of PCNA and Survivin proteins in the choriocarcinoma cell line（ＢｅＷｏ） during its fusion, so as to explore the proliferation change after the fusion of trophoblast cells. The study could be helpful for developing a new therapy of gestational trophoblastic tumor （ＧＴＴ）, especially drug-resistant GTT. Methods：The intercellular fusion of the cultured BeWo cells was induced by forskolin. The expression of Syncytin mRNA in the treated BeWo cells was detected by RT-PCR, and the expressions of PCNA and Survivin proteins were detected by Western blotting. The proliferation of BeWo cells was detected by MTT. Results：①The expression of Syncytin gene in the BeWo cell treated with forskolin was increased with a time-dependent manner, which reached its peak in 48 hours. ②The expressions of PCNA and Survivin proteins in BeWo cell after treatment were significantly decreased. ③The proliferation of the BeWo cell treated with forskolin was significantly decreased with a time-dependent manner. Conclusions：The lowered expressions of PCNA and Survivin proteins in those fusion choriocarcinoma cells means the lowered proliferation, suggesting that the induced intercellular fusion of trophoblast cells may be helpful to treat those malignant GTT.

Key words: Proliferating cell nuclear antigen, Microtubule-associated proteins, Forskolin, Cell fusion