Abstract: Objective：To explore the effect of exogenous prostaglandin E2 on the embryonic adhesion rate by integrin αv and β3, as well as the potential mechanism. Methods：The expressions of integrin αv and β3 mRNA and protein in the cultured RL95-2 cells treated with exogenous PGE2 were detected by real-time PCR and Western blotting. BeWo trophoblast cells and RL95-2 endometrial epithelial cells were used to establish the in vitro model. The RL95-2 epithelial cell monolayer was pretreated by different concentrations of PGE2. The rate of the adhesion between BeWo cell and RL95-2 epithelial cell monolayer was compared. Results： PGE2 （200 nmol/L） significantly increased the expressions of integrin αv mRNA and protein （P＜0.05）, while PGE2 （100 nmol/L） also significantly increased the expressions of integrin β3 mRNA and protein in RL95-2 cells （P＜0.0５）. The rate of the BeWo sphere cell adhered to the RL95-2 cell monolayer was significantly increased by the pretreatment with 200 nmol/L PGE2. Conclusions： PGE2 in appropriate concentration may increase the expressions of integrin αv and β3, and improve the embryonic adhesion. This study provides further evidence to promote the embryonic adhesion during implantation by increasing the expression of integrin αv and β3 and improving the endometrial receptivity.

Key words: Integrin alphaV, Integrin beta3, Prostaglandins E, Embryo implantation