Abstract: Objective：To investigate the influential factors on the clinical outcomes of artificial insemination with donor sperm (AID). Methods: The data of 4 067 AID cycles from 1 810 couples between January 2012 and April 2017 were retrospectively analyzed, to investigate the effects of women′s age, female infertility factors, therapeutic regimen, insemination timing and the numbers of treatment cycle on the AID outcomes. Results: ①Both clinical pregnancy rates and live birth rates were decreased with women′s age (both P＜0.05).②Women with ovulation failure, postoperative pelvic endometriosis or pelvic inflammatory disease had comparable clinical outcomes to those infertile women with pure male factors of husbands (all P＞0.05）. ③There were no significant differences in clinical pregnancy rate, abortion rate or live birth rate among natural cycle, clomiphene cycle, letrozole cycle and pure Gn cycle (both P＞0.05), while the rates of multiple pregnancy in clomiphene and letrozole cycle were significantly higher than that in natural cycle (both P＜0.001). ④Insemination before or after ovulation didn′t affect the clinical outcomes of AID in both natural cycle and ovulation induction cycle (both P＞0.05). ⑤Both cumulative pregnancy rate and cumulative live birth rate were significantly increased with the numbers of treatment cycle within the first 4 cycles (both P＜0.05), but no longer increased visibly since the fifth cycle (both P＞0.05). Conclusions: Women′s age was an important factor of the success of AID. We found in this paper that other factors of female infertility and insemination timing could not significantly affect the clinical outcomes of AID. IVF treatment should be considered for those women who did not get pregnancy after 3 or 4 AID treatment cycles, or those women aged over 40 years.

Key words: Artificial insemination with donor sperm, Pregnancy rate, Abortion, Live birth, Pregnancy outcome