Abstract: It has been demonstated that sperm DNA integrity，mainly caused by oxidative stress，
abnormal package of chromatin and abortive apoptosis，have a negative relationship with some semen
evaluation parameters such as concentration，motility and morphology，and could affect the potential of
fertilization and embryonic development，the outcomes of assisted reproductive technology，and increase the
risk of genetic disease for off鄄spring. Cryopreservation of human spermatozoa and sperm preparation techniques
can influence the integrity of sperm DNA. This article briefly reviews the mechanism of sperm DNA damage，
cryopreservation，sperm preparation techniques and the relationship of sperm DNA damage and ART.

Key words: Reproductive techniques, assisted, Sperm, DNA damage, Semen preservation, Cryop鄄 reservation, Pregnancy outcome