Abstract: Objective：To develop a stable beagle model of intrauterine adhesions（IUA） by mechanical curettage. Methods：The uterine tissues from six sexual maturity and healthy female beagle dogs were divided into four groups：Group 1，distal endometrial samples of the right uterus from those beagle dogs treated by mechanical curettage method, after two months；Group 2，distal samples of the left uterus without treatment；Group 3，proximal samples of the right uterus without treatment；Group 4，proximal samples of the left uterus without treatment. Samples were analyzed by HE staining, Masson staining and immunohistochemistry. Results：The incidence of abnormal endometrial appearance in the Group 1 was obviously higher than that of the Group 2（P＜0.05）. The endometrial thickness of the Group 1 was obviously thinner than those of Group 3 and Group 2（P＜0.05）. The number of endometrial gland of the Group 1 was significantly less than those of other groups（P＜0.05），while the area of endometrial fibrosis was also significantly bigger（P＜0.001）. The expression levels of IGF-Ⅰ and TGF-β1 in the Group 1 were significantly higher than those in other three groups（P＜0.05），while the expression level of bFGF was significantly higher than that in the Group 2（P＜0.05）. Conclusions：A stable beagle model of IUA was successfully developed by the mechanical curettage. The increased expressions of IGF-Ⅰ，bFGF and TGF-β1 may be involved in IUA in this model.

Key words: Adhesions, Dilatation and curettage, Models, animal, Canidae