Abstract: As there is an increase of researches concerning andrology，the assessment of sperm has
come to the cellular and molecular level. Sperm chromatin integrity is a new marker of male fertility. It affects
the fertilization，embryo quality，pregnancy and so on. Accordingly，it is necessary to detect sperm chromatin
integrity and adopt certain measures to obtain those sperm with less or no damage，which this can improve the
outcome of ART. Sperm chromatin structure assay（SCSA）is one of the common techniques to measure sperm
chromatin integrity. This review focuses on the mechanism and detection of sperm chromatin integrity damage，
its association with reproductive outcomes，and relevant treatment strategies in assisted reproductive
technology

Key words: Spermatozoa, DNA, DNA damage, Reproductive techniques, assisted, Infertility, male, Sperm injections, intracytoplasmic