Abstract: Ideal gene vector should be non-toxic, efficient, targeted and easy preparation, low price, etc. Viral and nonviral vectors are commonly used now. Those novel non -viral vectors including inorganicnanoparticles are expected to be developed into the ideal gene carriers. Inorganic nanoparticles as gene carrierscan be used to wrap, concentrat, and protect the nucleic acid from nuclease degradation, and to mediate geneexpression in target cells. Nanoparticles vectors are characterized by almost no toxicity, easy preparation,preservation convenient, large surface area, good biological compatibility and biodegradability, and stability. Ithas been indicated that the inorganic nanoparticles as nonviral gene dilivery vector has broad applicationporspects, based on systematic review and investigation on published literature in this field. We are trying touse this technology toa variety of genes or gene fragments, such as Calretinin gene and its siRNA into multiple gene fragments.

Key words: Nanoparticles, Genetic vectors, Gene therapy, Equipment safety