Abstract:

In vitro culture and cryopreservation of oocytes are two critical steps of assisted reproductive technology, and oxidative stress is a major limiting factor affecting the developmental quality of oocytes during above steps. At present, the commonly used natural antioxidants in clinical practice (such as melatonin, resveratrol, etc.) can alleviate oxidative damage, but the application effect is limited by inherent defects such as poor water solubility, low bioavailability and short metabolic half-life. In recent years, the rapid development of nanomaterial technology has provided new solutions to this challenge. Notably, nanoparticle-based antioxidant systems, owing to their unique free radical scavenging capacity and excellent biocompatibility, have been demonstrated significant advantages in in vitro manipulation of gamete. This article reviews the application progress of nanoparticles in the field of assisted reproduction, including the biological effects, potential mechanisms of reproductive toxicity, clinical prospects and future directions.

Key words: Nanoparticles, Reproductive techniques, assisted, In vitro techniques, Cryopreservation, Vitrification