Abstract: Clinical data of human assisted reproduction have shown that the assisted reproductive technology（ART） is associated with spontaneous abortion，premature birth ， perinatal death and low birth weight children，as well as some diseases related to imprinting genes. During gamete and pre-implantation embryo development, any mistakes of genomic imprinting, including erasure, re-establishment and maintenance, would lead defects of embryonic development, even embryonic death. ART manipulation is just in the process of imprinting genes. So, those abnormal outcomes of ART could be related with abnormal expression of imprinted genes. The main manipulation of ART, including ovulation induction, in vitro fertilization（IVF）， intracytoplasmic spern injection（ICSI）, cell biopsy for PGD, and in vitro culture, could influence the gene expression and phenotype， thus affect the growth and development potential of offspring by disturbing the reconstruction and maintenance of genomic imprinting.

Key words: Reproductive techniques, assisted, Genes, Genetic markers, DNA methylation, Genomic imprinting