Abstract: Sperm morphology is closely related to male fertility. Abnormal sperm morphology is one of main causes of male infertility. Since the intracytoplasmic sperm injection（ＩＣＳＩ） was successfully developed in 1992, it has helped so many infertile couples with abnormal sperm parameters to obtain their children. Under a magnification of ×400, the only major defects in sperm morphology could be found. Those sperms with many abnormities in subcellular organelles might not be identified, which could affect the success rate of ICSI. The motile sperm organelle morphology examination（ＭＳＯＭＥ） established by Bartoov in 2001 allowed to assess the subcellular organelles of sperm under high magnification, which is helpful to pick out sperm with normal morphology. The intracytoplasmic morphologically selected sperm injection（ＩＭＳＩ）, combined MSOME with ICSI，provided a new technique for the clinical ICSI practice.

Key words: Spermatozoa, Sperm injections, intracytoplasmic, Motile sperm organelle morphology examination, Vacuoless