国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2010, Vol. 29 ›› Issue (4): 252-255.

• 论著 • 上一篇    下一篇

胰岛素基因可变重复序列多态性与PCOS的相关性

张琴芬,韩素萍,崔毓桂,李瑛,马翔,高莉,冒韵东,刘嘉茵   

  1. 210009 东南大学附属中大医院妇产科（张琴芬）；南 京医科大学第一附属医院生殖医学科（韩素萍，崔毓桂，马翔，高莉， 冒韵东，刘嘉茵）；江苏省计划生育科学技术研究所（李瑛）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-07-15 发布日期:2010-07-15
  • 通讯作者: 刘嘉茵,韩素萍

Polymorphism of Insulin Gene Variable Number Tandem Repeats Correlation with Polycystic OvarySyndrome

ZHANG Qin-fen，HAN Su-ping，CUI Yu-gui，LI Ying，MA Xiang，GAO Li，MAO Yun-dong， LIU Jia-yin   

  1. Department of Obstetrics and Gynecology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, China（ZHANG Qin-fen）；Center of Clinical Reproductive Medicine，First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China（HAN Su-ping，CUI Yu-gui，MA Xiang，GAO Li， MAO Yun-dong，LIU Jia-yin)；Jiangsu Family Planning Institute，Nanjing 210036，China(LI Ying)
  • Received:1900-01-01 Revised:1900-01-01 Published:2010-07-15 Online:2010-07-15
  • Contact: LIU Jia-yin,fen，HAN Su鄄ping

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摘要： 目的：探讨胰岛素基因可变重复序列（INS-VNTR）多态性与多囊卵巢综合征（PCOS）易感
性之间的关系及其与PCOS 发生胰岛素抵抗（IR）的相关性。方法：用聚合酶链反应－限制性片段多态性技术，检测130 例PCOS 患者(PCOS 组)和130 例非PCOS 妇女(对照组)的INS-VNTR 多态性。结果：2 组的I/ I、I/III芋和III/III基因型频率分布差别无统计学意义（P=0.196），经Logistic 回归分析，III/III基因型并没有 增加PCOS 发病的危险性（OR=6.25，95% CI:0.74~52.79）。PCOS 胰岛素抵抗亚组(PCOS-IR 组，88 例)相应
位点的INS-VNTR基因型频率及等位基因频率均高于PCOS 非胰岛素抵抗亚组（PCOSNIR 组，42 例， P<0.05)；PCOS 患者中芋/芋基因型者的INS、IR 状态高于I/I基因型者。结论：VNTR 可能不是PCOS 发病 的易感基因，但是Ⅲ型INS-VNTR 可能使PCOS 患者发生IR 的危险性增加。

关键词: 多囊卵巢综合征, 胰岛素, 多态现象, 遗传, 胰岛素抗药性, 聚合酶链反应, 多态性, 限 制性片段长度, 串联重复序列

Abstract: Objective：To investigate the correlation between the insulin gene variable number tandem
repeats（INS鄄VNTR）and polycystic ovary syndrome（PCOS）and metabolic features related to insulin resistance
（IR）. Methods：One hundred and thirty patients with PCOS（PCOS group）and 130 normal women（control
group）were included. Genotyping of INS-VNTR was performed using polymerase chain reaction-restriction
fragment length polymorphism（PCR-RFLP）. Results：The distribution of genotype of INS-VNTR was similar in
PCOS group，and compared with the I/Icarriers, an odd ratio （OR）for III/IIIcarriers was 6.25 [95%
confidence interval（CI）=0.749-52.79]. The distribution of genotype and the 芋allele frequency of INS-VNTR
in PCOS insulin resistance（PCOS-IR）group were significantly higher than that in PCOS non-insulin resistance
group （PCOS-NIR）. Moreover, PCOS women with III/IIIhad statistically significantly higher fasting insulin
lever and HOMA-IR than that of PCOS women with I/I. Conclusions：INS-VNTR may not be a susceptibility
gene，but INS VNTR polymorphism may play an important role in the occurrence of insulin resistance in
patients with PCOS.

Key words: Polycystic ovary syndrome, Insulin, Polymorphism, genetic, Insulin resistance, Polymerase chain reaction, Polymorphism, restriction fragment length, Tandem repeat sequences