国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2011, Vol. 30 ›› Issue (5): 365-367.

• 论著 • 上一篇    下一篇

硫酸普拉睾酮钠联合链脲佐菌素诱导大鼠PCOS伴 2型糖尿病模型的实验研究

李 维 ,李赛姣, 尹太郎, 杨 菁   

  1. 430060 武汉大学人民医院生殖医学中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-09-15 发布日期:2011-09-15
  • 通讯作者: 杨 菁

Animal Model of PCOS with Type 2 Diabetes Induced by Sodium Prasterone Sulfate and Streptozotocin

LI Wei， LI Sai-jiao， YIN Tai-lang，YANG Jing   

  1. Reproductive Medicine Center， Renmin Hospital of Wuhan University， Wuhan 430060， China
  • Received:1900-01-01 Revised:1900-01-01 Published:2011-09-15 Online:2011-09-15
  • Contact: YANG Jing

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摘要： 目的：研究采用硫酸普拉睾酮钠及高脂高糖饲料+链脲佐菌素（STZ）诱导PCOS伴2型糖尿病（2-DM）大鼠模型的实验方法。方法：将23日龄雌性SD大鼠分为PCOS组，PCOS伴2-DM组及对照组，PCOS组以硫酸普拉睾酮钠90 μg/（g·d）皮下注射 20 d，同时给予高脂高糖饲料喂养；PCOS伴2-DM组则在硫酸普拉睾酮钠停药后第2天给予STZ 45 mg/kg腹腔注射1次，PCOS组及对照组注射等量柠檬酸盐缓冲液。注射后72 h处死大鼠，观察卵巢外观、光镜（HE染色）改变，测定血清睾酮（T）、雌二醇（E2）及血糖、胰岛素水平，计算HOMA?鄄IR指数。结果：PCOS伴2-DM组与PCOS组大鼠卵巢均呈多囊样改变，血清T、E2浓度均显著高于对照组（P＜0.01） ，PCOS伴2-DM组血糖浓度明显高于PCOS组及对照组（P＜0.01），且HOMA?鄄IR指数与对照组相比明显增高，差异有统计学意义（P＜0.05）。结论：硫酸普拉睾酮钠联合高脂高糖饲料+低剂量STZ诱导SD 幼年雌性大鼠的PCOS伴2-DM的动物模型，表现出与PCOS伴2-DM患者相似的内分泌特点，可用于PCOS远期并发症的研究，是较理想的动物模型。

关键词: 多囊卵巢综合征, 糖尿病, 2型, 硫酸脱氢表雄酮, 糖尿病, 实验性, 疾病模型, 动物

Abstract: Objective： To develop a rat model of polycystic ovarian syndrome（PCOS） with type 2 diabetes（2-DM） induced by sodium prasterone sulfate and streptozotocin（STZ）. Methods： Female rats aged 23-day were injected daily S. C. with sodium prasterone sulfate 90 μg/（g·d） for 20 days, then intraperitoneally injected streptozotocin once in a dose of 45 mg/kg. All rats were feed with high fat and sugar feed. The ovarian weight and pathological morphology was observed. The sera levels of testosterone（T），estradiol（E2）， luteinizing hormone（LH）， follicle-stimulating hormone（FSH）， blood glucose（BG） and insulin（INS） were measured. Results： The ovaries of both PCOS group and the PCOS with 2-DM group showed multiple follicular cysts. Ovarian weight, sera T, E2 and INS of two groups were significantly higher than those of control group（P＜0.01）. BG concentration of the PCOS with 2-DM group was significantly higher than those of PCOS group and control group（P＜0.01）， while HOMA index of the PCOS with 2-DM group was higher that that of control group（P＜0.05）. Conclusions： The rat model of PCOS with 2-DM showed the morphologic and hormonal changes which were similar to patients with PCOS with 2-DM. This animal model induced by the sodium prasterone sulfate and streptozotocin is a good model of PCOS with 2-DM for studying the long?鄄term complications of PCOS.

Key words: Polycystic ovary syndrome, Diabetes mellitus, type 2, Dehydroepiandrosterone sulfate, Diabetes mellitus, experimental, Disease models, animal