国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2014, Vol. 33 ›› Issue (2): 99-102.

• 论著 • 上一篇    下一篇

利用IVF结局评估胚胎培养系统的稳定性

纪冬梅，章志国，周平，魏兆莲，陈蓓丽，郝燕，陈大蔚，曹云霞   

  1. 230022 合肥，安徽医科大学第一附属医院生殖医学中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-03-15 发布日期:2014-03-15
  • 通讯作者: 曹云霞

Evaluation of the Stability of Embryo Culture System by IVF Outcomes

JI Dong-mei，ZHANG Zhi-guo，ZHOU Ping，WEI Zhao-lian，CHEN Bei-li，HAO Yan，CHEN Da-wei，CAO Yun-xia   

  1. Center of Reproductive Medicine，the First Affiliated Hospital of Anhui Medical University，Hefei 230022，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2014-03-15 Online:2014-03-15
  • Contact: CAO Yun-xia

PDF (PC)

7483

摘要： 目的：分析不同批次卵裂期胚胎培养液所获得的体外受精（IVF）结局，以进一步评估不同批次培养液的稳定性。方法：病例资料为本中心2011年8月—2012年9月患者年龄＜35岁、卵巢储备功能正常、采用常规长方案促排卵并且获卵数＞5枚的移植周期，根据卵裂期培养液的批次不同收集了18组，随机抽取6组，分析各组患者一般情况及IVF结局。结果：6组患者年龄、既往IVF次数、基础卵泡刺激素（FSH）水平、体质量指数（BMI）和人绒毛膜促性腺激素（hCG）日子宫内膜厚度比较差异无统计学意义（均P＞0.05）。各组平均获卵数、卵子成熟率、受精率和卵裂率差异无统计学意义（均P＞0.05），优质胚胎率差异有统计学意义（P＜0.05）。各组间平均移植胚胎数、妊娠率、种植率和流产率差异无统计学意义（均P＞0.05）。结论：本中心IVF实验室整体质控系统稳定，不同批次培养液的IVF结局稳定。

关键词: 生殖技术, 辅助, 受精, 体外, 胚胎移植, 质量控制

Abstract: Objective: To further evaluate the stability of different batches of culture medium by analyzing the IVF outcomes. Methods：Clinical data were collected from August 2011 to September 2012 in our IVF centre. Patients′ characteristics were summarized as younger than 35 years，normal ovarian reserve, using long protocol for ovulation, and more than 5 oocytes per cycles. There were 18 batches of the culture medium according to the cleavage stage （１８ ｇｒｏｕｐｓ）． Six batches （６ ｇｒｏｕｐｓ） were randomly selected. General clinical features and IVF outcomes were compared. Results：There were no significant differences in the average age，the number of previous IVF，basal FSH level，body mass index （BMI）, and endometrial thickness of hCG day, among the six groups（P＞0.05）. There were no significant differences in the average number of oocyte，oocyte maturation rate，fertilization rate，cleavage rate（P＞0.05） among the groups. The high-quality embryo rate was different among the groups（P＜0.05）. However，there were no significant differences in the average number of embryos transferred，pregnancy rate，miscarriage rate among the groups（P＞0.05）. Conclusions：IVF outcomes from different batches of the embryonic culture medium are stable, suggesting the stability of the overall quality control system in our IVF laboratory.

Key words: Reproductive techniques, assisted, Fertilization in vitro, Embryo transfer, Quality control